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介电泳悬浮法测定的介电性能与细胞在电场中暴露的生长活性之间的相关性。

Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field.

机构信息

Department of Chemical and Environmental Engineering, Gunma University, 1-5-1 Tenjin Cho, Kiryu, Gunma 376-8515, Japan.

出版信息

Bioprocess Biosyst Eng. 2013 Sep;36(9):1219-27. doi: 10.1007/s00449-012-0849-3. Epub 2012 Nov 20.

DOI:10.1007/s00449-012-0849-3
PMID:23178982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3755225/
Abstract

The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Re[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.

摘要

本研究旨在开发一种通过介电泳方法分析细胞活性的系统。我们之前的研究表明，通过介电泳悬浮，小鼠杂交瘤 3-2H3 细胞的生长活性与介电特性（Re[K(ω)])之间存在相关性。此外，还明确表明无需诱导分化，许多干细胞的分化活性可以通过 Re[K(ω)]来评估。在本文中，培养了暴露于交流（AC）电场或直流（DC）电场的 3-2H3 细胞，并检查了电场对细胞生长活性的损伤影响。为了通过测量 Re[K(ω)]来评估细胞的活性，检查了暴露于电场的细胞的生长活性与 Re[K(ω)]之间的相关性。获得了暴露于 AC 电场的细胞的细胞活力、生长活性和 Re[K(ω)]之间的关系。可以通过 Re[K(ω)]评估暴露于 AC 电场的细胞的生长活性。此外，发现与 DC 电场相比，AC 电场对细胞活力和生长活性的不利影响较小。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/db976ff961c7/449_2012_849_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/ea5b4e866885/449_2012_849_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/d870a363886f/449_2012_849_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/21eca80964b1/449_2012_849_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/7f68ecc6b973/449_2012_849_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/7ddad368a49b/449_2012_849_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/9ddf621ae77c/449_2012_849_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/b61a485bd66d/449_2012_849_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/dc62c5980422/449_2012_849_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/d0bdd9309c63/449_2012_849_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/488a2f969d7a/449_2012_849_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/aa0ee8d9ad3e/449_2012_849_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/41bb4c970878/449_2012_849_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/52a0ef0fdfc0/449_2012_849_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/db976ff961c7/449_2012_849_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/ea5b4e866885/449_2012_849_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/d870a363886f/449_2012_849_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/21eca80964b1/449_2012_849_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/7f68ecc6b973/449_2012_849_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/7ddad368a49b/449_2012_849_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/9ddf621ae77c/449_2012_849_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/b61a485bd66d/449_2012_849_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/dc62c5980422/449_2012_849_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/d0bdd9309c63/449_2012_849_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/488a2f969d7a/449_2012_849_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/aa0ee8d9ad3e/449_2012_849_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/41bb4c970878/449_2012_849_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/52a0ef0fdfc0/449_2012_849_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3656/3755225/db976ff961c7/449_2012_849_Fig14_HTML.jpg

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