Yoshida Akira, Isomoto Hajime, Hisatsune Junzo, Nakayama Masaaki, Nakashima Yujiro, Matsushima Kayoko, Mizuta Yohei, Hayashi Tomayoshi, Yamaoka Yoshio, Azuma Takeshi, Moss Joel, Hirayama Toshiya, Kohno Shigeru
Second Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto, Nagasaki 852-8501, Japan.
Clin Immunol. 2009 Mar;130(3):290-7. doi: 10.1016/j.clim.2008.09.016. Epub 2008 Nov 8.
CC chemokine ligand 20 (CCL20) attracts CC chemokine receptor 6 (CCR6)-expressing cells. Using endoscopic biopsies taken from the gastric antrum of 42 subjects infected with H. pylori and 42 uninfected subjects, mucosal CCL20 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. CCL19 mRNA and protein levels, as well as CCL21 mRNA levels, were also measured. The CCL20 mRNA and protein levels were significantly elevated in H. pylori-positive patients and substantially decreased after successful eradication. CCL19 and CCL21 expression levels were comparable in the H. pylori-infected and the uninfected groups. The CCL20 concentrations correlated with the degree of chronic gastritis. Immunohistochemistry and the in vitro infection assay showed that CCL20 was principally produced by the gastric epithelium. CCR6-expressing cells, including CD45RO(+) memory T lymphocytes and fascin(+)-CD1a(+) immature dendritic cells, infiltrated close to the CCL20-expressing epithelial cells. The CCL20/CCR6 interaction may be involved in the development of H. pylori-associated gastritis.
C-C趋化因子配体20（CCL20）可吸引表达C-C趋化因子受体6（CCR6）的细胞。利用42名幽门螺杆菌感染受试者和42名未感染受试者的胃窦内镜活检样本，分别通过实时聚合酶链反应和酶联免疫吸附测定法检测黏膜CCL20 mRNA和蛋白水平。同时也检测了CCL19 mRNA和蛋白水平以及CCL21 mRNA水平。幽门螺杆菌阳性患者的CCL20 mRNA和蛋白水平显著升高，成功根除幽门螺杆菌后大幅下降。CCL19和CCL21的表达水平在幽门螺杆菌感染组和未感染组中相当。CCL20浓度与慢性胃炎程度相关。免疫组织化学和体外感染试验表明，CCL20主要由胃上皮细胞产生。表达CCR6的细胞，包括CD45RO(+)记忆T淋巴细胞和fascin(+)-CD1a(+)未成熟树突状细胞，浸润至表达CCL20的上皮细胞附近。CCL20/CCR6相互作用可能参与幽门螺杆菌相关性胃炎的发生发展。
