Wang Lin, Buchanan Stacey, Meyerhoff Mark E
Anal Chem. 2008 Dec 15;80(24):9845-7. doi: 10.1021/ac801879t.
A very simple and inexpensive method to detect oversulfated chondroitin sulfate (OSCS) or other high-charge density polyanionic structures as contaminants in heparin products using potentiometric polyanion sensors is described. In the potentiometric measurement, a greater change in the phase boundary equilibrium potential of polymeric membranes formulated with tridodecylmethylammonium (TDMA) anion exchange sites is observed for the contaminated heparin than for the untainted heparin due to the higher charge density of OSCS or other impurities compared to that of porcine heparin. Detection of 0.5 wt % OSCS impurity is readily achieved using only 1 mg/mL of final polyion concentration. Even lower detection limits for OSCS contamination may be possible if higher final concentrations of heparin preparations can be employed in the test procedure.
描述了一种非常简单且廉价的方法，即使用电位型聚阴离子传感器检测硫酸皮肤素（OSCS）或其他高电荷密度的聚阴离子结构作为肝素产品中的污染物。在电位测量中，由于OSCS或其他杂质的电荷密度高于猪肝素，因此与未受污染的肝素相比，受污染的肝素会使含有三（十二烷基）甲基铵（TDMA）阴离子交换位点的聚合物膜的相界平衡电位发生更大的变化。仅使用1mg/mL的最终聚离子浓度即可轻松检测出0.5wt%的OSCS杂质。如果在测试过程中可以使用更高浓度的肝素制剂，则可能实现更低的OSCS污染检测限。
