Wang Lin, Meyerhoff Mark E
Department of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109-1055.
Electroanalysis. 2010 Jan;22(1):26-30. doi: 10.1002/elan.200900434.
Quantification of oversulfated chondroitin sulfate (OSCS) in biomedical heparin preparations is achieved using a recently described potentiometric polyanion sensor-based approach operated in a kinetic mode of analysis. This is accomplished by adjusting the concentration of the test sample to a range where the OSCS level is low enough for the sensor not to achieve a full and rapid equilibrium phase boundary potential change at the membrane/sample interface upon exposure to the heparin sample. Using this method, the OSCS wt% determined within heparin samples containing OSCS are shown to be in good agreement with those determined by an accepted NMR method.
使用最近描述的基于电位聚阴离子传感器的方法，以动力学分析模式实现生物医学肝素制剂中过硫酸化硫酸软骨素（OSCS）的定量。这是通过将测试样品的浓度调整到一个范围来实现的，在该范围内，OSCS水平足够低，以至于传感器在暴露于肝素样品时，在膜/样品界面不会实现完全且快速的平衡相界电位变化。使用这种方法，含有OSCS的肝素样品中测定的OSCS重量百分比与通过公认的核磁共振方法测定的结果高度一致。
