Khanna Kamal M, Aguila Carolina C, Redman Jason M, Suarez-Ramirez Jenny E, Lefrançois Leo, Cauley Linda S
Department of Immunology, University of Connecticut, Farmington, CT 06032-1319, USA.
Eur J Immunol. 2008 Dec;38(12):3304-15. doi: 10.1002/eji.200838602.
Pulmonary influenza infection causes prolonged lymph node hypertrophy while processed viral antigens continue to be presented to virus-specific CD8 T cells. We show that naïve, but not central/memory, nucleoprotein (NP)-specific CD8 T cells recognized antigen-bearing CD11b(+) DC in the draining lymph nodes more than 30 days after infection. After these late transfers, the naïve CD8 T cells underwent an abortive proliferative response in the mediastinal lymph node (MLN), where large clusters of partially activated cells remained in the paracortex until at least a week after transfer. A majority of the endogenous NP-specific CD8 T cells that were in the MLN between 30 and 50 days after infection also showed signs of a continuing response to antigen stimulation. A high frequency of endogenous NP-specific CD8 T cells in the MLN indicates that late antigen presentation may help shape the epitope dominance hierarchy during reinfection.
肺部流感感染会导致淋巴结长期肥大，同时处理后的病毒抗原会持续呈递给病毒特异性CD8 T细胞。我们发现，在感染后30多天，幼稚而非中央/记忆性核蛋白（NP）特异性CD8 T细胞在引流淋巴结中识别携带抗原的CD11b(+)树突状细胞（DC）。在这些晚期转移后，幼稚CD8 T细胞在纵隔淋巴结（MLN）中经历了一次流产性增殖反应，在那里，大量部分活化的细胞簇留在副皮质区，直到转移后至少一周。感染后30至50天在MLN中的大多数内源性NP特异性CD8 T细胞也显示出对抗原刺激持续反应的迹象。MLN中高频率的内源性NP特异性CD8 T细胞表明，晚期抗原呈递可能有助于在再次感染期间塑造表位优势等级。
