Box Antonio, Sureda Antoni, Deudero Salud
Marine Biology Laboratory, University of the Balearic Islands, Palma de Mallorca, Balearic Islands, Spain.
Comp Biochem Physiol C Toxicol Pharmacol. 2009 May;149(4):456-60. doi: 10.1016/j.cbpc.2008.10.107. Epub 2008 Nov 3.
Invasive species represent a risk to natural ecosystems and a biodiversity hazard. The present work aims to determine the antioxidant enzyme response - superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the phase II detoxifying enzyme - glutathione S-transferase (GST) - and markers of oxidative damage - thioredoxin reductase (TR) and malondialdehyde (MDA) - in gills and digestive gland of Pinna nobilis and to study the antioxidant response effects in the bivalve colonised by the invasive macroalgae Lophocladia lallemandii. Colonised specimens were collected in a control area without L. lallemandii and another area completely colonised by L. lallemandii. All enzyme activities were found to be present in gills and digestive gland, with some tissue differences. CAT and SOD activities were higher in gills than digestive gland, whereas GST activity and MDA levels were higher in digestive gland. The presence of L. lallemandii induced a significant increase in the activities of antioxidant enzymes in both gills and digestive gland, except for CAT activity in gills. GST and TR activities were also increased in both tissues, as well as the MDA concentration. We can conclude that the presence of L. lallemandii colonising P. nobilis induces a biological stress and oxidative damage to the fan mussel.
入侵物种对自然生态系统构成风险，也是生物多样性的一大危害。本研究旨在测定华贵栉孔扇贝鳃和消化腺中的抗氧化酶反应——超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）、II期解毒酶——谷胱甘肽S-转移酶（GST）以及氧化损伤标志物——硫氧还蛋白还原酶（TR）和丙二醛（MDA），并研究被入侵大型海藻拉氏黑顶藻定殖的双壳贝类中的抗氧化反应效应。在一个没有拉氏黑顶藻的对照区域和另一个完全被拉氏黑顶藻定殖的区域采集定殖样本。结果发现所有酶活性在鳃和消化腺中均有存在，且存在一些组织差异。鳃中的CAT和SOD活性高于消化腺，而消化腺中的GST活性和MDA水平更高。拉氏黑顶藻的存在导致鳃和消化腺中的抗氧化酶活性显著增加，但鳃中的CAT活性除外。两个组织中的GST和TR活性以及MDA浓度也都增加了。我们可以得出结论，拉氏黑顶藻定殖在华贵栉孔扇贝上会对栉孔扇贝造成生物胁迫和氧化损伤。
