Faculty of Veterinary Science, The University of Sydney, PMB 3, Camden NSW, 2570, Australia.
Vet J. 2010 Feb;183(2):217-8. doi: 10.1016/j.tvjl.2008.09.018. Epub 2008 Nov 17.
Increasing interest in the role of oxidative stress (OS) in equine medicine has highlighted the need to develop reliable methods to quantify it. In this study we describe the effect of refrigeration (at 4 degrees C) on the stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests carried out on 15 healthy horses. Blood samples, collected from the jugular vein, were immediately placed on ice and analysed using both the d-ROMs and BAP tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 h. The average results were similar for up to 24 h and minimal variations were found for each horse. The findings suggest that refrigeration is suitable for preserving equine blood samples for these assays and this approach will provide veterinarians with a technically simple, reliable test to measure OS under field conditions.
人们对氧化应激（OS）在马医学中作用的兴趣日益增加，这凸显了开发可靠方法来对其进行定量分析的必要性。在这项研究中，我们描述了冷藏（4°C）对 15 匹健康马的活性氧代谢物（d-ROMs）和生物抗氧化潜力（BAP）检测的稳定性的影响。从颈静脉采集的血液样本立即置于冰上，并使用 d-ROMs 和 BAP 检测进行分析。样本还在 4°C 下冷藏，并在 3、7 和 24 小时后进行测试。在 24 小时内，平均结果相似，并且每匹马的变化都很小。这些发现表明，冷藏适用于保存这些检测的马血液样本，这种方法将为兽医提供一种在现场条件下测量 OS 的技术简单、可靠的检测方法。
