Soares Betânia Maria, da Silva Danielle Letícia, Sousa Gerdal Roberto, Amorim José Cláudio Faria, de Resende Maria Aparecida, Pinotti Marcos, Cisalpino Patrícia Silva
Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais, UFMG, Av. Antônio Carlos, 6627-CEP.: 31270-901, Belo Horizonte, Minas Gerais, Brazil.
J Photochem Photobiol B. 2009 Jan 9;94(1):65-70. doi: 10.1016/j.jphotobiol.2008.07.013. Epub 2008 Aug 30.
In this study, photodynamic inactivation (PDI) was used to inhibit in vitro growth and adhesion of different Candida isolates to buccal epithelial cells (BEC). Experimental conditions were optimized and 25muM toluidine blue O (TBO) and 15min of irradiation time by light emitting diode (LED) (energy density of 180J/cm(2)) were selected due to higher reductions in cellular viability obtained after treatment. Reduction media of Log(10) 3.41 in viable cellular growth and media of 55% in the inhibition of adhesion to buccal epithelial cells were obtained. Two fluconazole resistant isolates were susceptible to PDI (Log(10) 3.54 in IB05 and Log(10) 1.95 in CG09) and a second session of this treatment for CG09 isolate inhibited cellular viability in 100%, without producing heat. The results permit to conclude that photodynamic inactivation under these experimental conditions would be a possible alternative approach to inhibit Candida spp. cellular growth and adhesion to buccal epithelial cells.
在本研究中，光动力灭活（PDI）被用于抑制不同念珠菌分离株在体外的生长以及其对颊上皮细胞（BEC）的黏附。对实验条件进行了优化，由于处理后细胞活力有更高程度的降低，选择了25μM的甲苯胺蓝O（TBO）以及由发光二极管（LED）照射15分钟（能量密度为180J/cm²）。在活菌生长方面获得了Log(10) 3.41的降低幅度，在抑制对颊上皮细胞的黏附方面获得了55%的抑制率。两株氟康唑耐药分离株对PDI敏感（IB05为Log(10) 3.54，CG09为Log(10) 1.95），对CG09分离株进行的第二轮该处理可100%抑制细胞活力，且不产生热量。结果表明，在这些实验条件下，光动力灭活可能是一种抑制念珠菌属细胞生长及其对颊上皮细胞黏附的替代方法。
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