Besson F, Michel G
Laboratoire de Biochimie Microbienne (CNRS URA 1176), Université Calude Bernard Lyon I, Villeurbanne, France.
Microbios. 1991;65(262):15-21.
When calcium chloride was added to the culture medium of strains producing iturin or bacillomycin L, the antibiotic, normally excreted in the supernatant of the culture medium, was found in the cell pellet. This apparent inhibition of the antibiotic excretion was studied and it was demonstrated that CaCl2 precipitated the antibiotic after its excretion in the medium. The ability of various chloride salts to precipitate iturin and bacillomycin L was tested and the most effective salts were CaCl2 and MnCl2. Comparison of the compounds obtained by CaCl2 precipitation and by acid precipitation showed that, in the latter case, major antibiotics were accompanied by minor congeners resulting from modifications of genuine antibiotics.
当向产生伊枯草菌素或杆菌霉素L的菌株的培养基中添加氯化钙时，原本分泌到培养基上清液中的抗生素却出现在细胞沉淀中。对这种抗生素分泌的明显抑制进行了研究，结果表明氯化钙在抗生素分泌到培养基后将其沉淀。测试了各种氯化物盐沉淀伊枯草菌素和杆菌霉素L的能力，最有效的盐是氯化钙和氯化锰。比较通过氯化钙沉淀和酸沉淀获得的化合物发现，在后一种情况下，主要抗生素伴有由真正抗生素修饰产生的次要同系物。
