Salant H, Weingram T, Spira D T, Eizenberg T
Department of Parasitology, Hebrew University, Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.
Vet Parasitol. 2009 Jan 22;159(1):24-9. doi: 10.1016/j.vetpar.2008.10.011. Epub 2008 Oct 11.
An outbreak of Toxoplasmosis in a colony of squirrel monkeys (Saimiri sciureus) in Israel is described. Serological, pathological, and molecular findings of monkeys, as well as rodents and pigeons from the vicinity are summarized. Seventy-nine percent (19/24) of monkeys were T. gondii seropositive at titer 1:16 whilst 4% (1/24) were also seropositive at titer 1:64 using the Modified Agglutination Test (MAT). Eighty four percent (21/25) of rats were positive at titer 1:16 and 8% (2/25) of rats were positive at titer 1:32. DNA amplification of a 529bp repeated sequence of T. gondii was detected in the liver and lungs of all monkeys tested, 6/7 in myocardial extractions and 5/6 in brain extractions. Sequence analysis of the SAG2 locus disclosed that T. gondii detected was of Type III genotype. The source of disease was thought to be contamination of feed with infective feline oocysts. As a result of this study, the implementation of a program to capture and remove resident feral cats, to discontinue the feeding of stray cats, and to control rodent populations in the park was introduced.
本文描述了以色列一群松鼠猴（松鼠猴属）爆发弓形虫病的情况。总结了猴子以及附近啮齿动物和鸽子的血清学、病理学和分子学研究结果。使用改良凝集试验（MAT），79%（19/24）的猴子弓形虫血清学检测呈阳性，滴度为1:16，而4%（1/24）的猴子滴度为1:64时也呈血清学阳性。84%（21/25）的大鼠滴度为1:16时呈阳性，8%（2/25）的大鼠滴度为1:32时呈阳性。在所有检测的猴子的肝脏和肺中均检测到弓形虫529bp重复序列的DNA扩增产物，在心肌提取物中有6/7检测到，在脑提取物中有5/6检测到。SAG2基因座的序列分析表明，检测到的弓形虫为III型基因型。认为疾病来源是感染性猫卵囊污染饲料。作为这项研究的结果，实施了一项计划，即捕捉并清除当地的野猫，停止喂养流浪猫，并控制公园内的啮齿动物数量。
