Akin Okhan Kadir, Serdar Muhittin A, Cizmeci Zeynep, Genc Ozlem, Aydin Suleyman
Kecioren Research and Training Hospital, Clinical Chemistry and Laboratory Science, Ankara 06380, Turkey.
Biotechnol Appl Biochem. 2009 Jun;53(Pt 2):139-44. doi: 10.1042/BA20080188.
Urine analysis is one of the most common tests for assessing urinary-tract and kidney diseases. In recent years there have been new developments in the automation of this test. The objective of the present study was to compare the performances of two urine sediment analysers, namely LabUMat with UriSed (77 Elektronika Kft, Budapest, Hungary) and iQ200 (Iris Diagnostics, Chatsworth, CA, U.S.A.), with the KOVA method for manual urine measurement by evaluating the results in terms of similar parameters (cells or particles per lower-power field or high-power field). The results obtained using the UriSed and iQ200 analysers were more reproducible (7.1-30.2 and 14.9-35.4% respectively) than those obtained using the manual technique (17.9-44.4%). Significant correlations were established among the three techniques in the evaluation of leucocytes, erythrocytes and epithelial cells. Although the UriSed, iQ200 and visual-microscopic measurements were in agreement, confirmation of the results from automated methods by manual urine analyses is significantly useful, especially for pathological cases that were close to the limits of the techniques.
尿液分析是评估泌尿系统和肾脏疾病最常见的检测方法之一。近年来,该检测的自动化有了新进展。本研究的目的是通过根据相似参数(每低倍视野或高倍视野中的细胞或颗粒)评估结果,比较两种尿沉渣分析仪LabUMat与UriSed(匈牙利布达佩斯77 Elektronika Kft公司)和iQ200(美国加利福尼亚州查茨沃思市Iris Diagnostics公司)与用于手动尿液测量的KOVA方法的性能。使用UriSed和iQ200分析仪获得的结果比使用手动技术获得的结果(分别为17.9 - 44.4%)具有更高的可重复性(分别为7.1 - 30.2%和14.9 - 35.4%)。在评估白细胞、红细胞和上皮细胞时,三种技术之间建立了显著的相关性。尽管UriSed、iQ200和目视显微镜测量结果一致,但通过手动尿液分析对自动化方法的结果进行确认非常有用,特别是对于接近技术极限的病理病例。