Jayakumar R, Rajkumar M, Freitas H, Selvamurugan N, Nair S V, Furuike T, Tamura H
Faculty of Chemistry, Materials and Bioengineering and High Technology Research Centre, Kansai University, Suita, Osaka 564-8680, Japan.
Int J Biol Macromol. 2009 Jan 1;44(1):107-11. doi: 10.1016/j.ijbiomac.2008.10.010. Epub 2008 Nov 5.
Alginate/phosphorylated chitin (P-chitin) blend films were prepared by mixing of 2% of alginate and P-chitin in water and then cross-linked with 4% CaCl2 solution. The blended films were characterized by FT-IR. Then, the bioactivity of blend films was studied by biomimetic method in simulated body fluid solution (SBF) for 7, 14 and 21 days. After 7, 14 and 21 days and films were characterized by FT-IR and SEM studies. The SEM and FT-IR studies showed that the hydroxyapatite was formed on the surface of the blend films after 7, 14 and 21 days in the SBF solution. These studies confirmed that the alginate/P-chitin blend films are bioactive. Furthermore, the adsorption of Ni2+, Zn2+ and Cu2+ onto alginate/P-chitin blend films has been investigated. The parameters studied include the pH, contact time, and initial metal ion concentrations. The maximum adsorption capacity of alginate/P-chitin blend films for Ni2+, Zn2+ and Cu2+ at pH 5.0 was found to be 5.67, 2.85 and 11.7 mg/g, respectively. These results suggest that alginate/P-chitin blend films-based technologies may be developed for water purification and metal ions separation and enrichment.
通过将2%的海藻酸盐和磷酸化几丁质（P-几丁质）在水中混合，然后与4%的氯化钙溶液交联，制备了海藻酸盐/磷酸化几丁质（P-几丁质）共混膜。通过傅里叶变换红外光谱（FT-IR）对共混膜进行了表征。然后，采用仿生法在模拟体液溶液（SBF）中对共混膜的生物活性进行了7天、14天和21天的研究。在7天、14天和21天后，通过FT-IR和扫描电子显微镜（SEM）研究对膜进行了表征。SEM和FT-IR研究表明，在SBF溶液中7天、14天和21天后，共混膜表面形成了羟基磷灰石。这些研究证实了海藻酸盐/P-几丁质共混膜具有生物活性。此外，还研究了Ni2+、Zn2+和Cu2+在海藻酸盐/P-几丁质共混膜上的吸附情况。研究的参数包括pH值、接触时间和初始金属离子浓度。发现在pH 5.0时，海藻酸盐/P-几丁质共混膜对Ni2+、Zn2+和Cu2+的最大吸附容量分别为5.67、2.85和11.7 mg/g。这些结果表明，可以开发基于海藻酸盐/P-几丁质共混膜的技术用于水净化以及金属离子的分离和富集。
