Moens Cecilia B, Donn Thomas M, Wolf-Saxon Emma R, Ma Taylur P
HHMI and Division of Basic Science, Fred Hutchinson Cancer Research Center, B2-152, 1100 Fairview Ave. N., Seattle, WA 98109, USA.
Brief Funct Genomic Proteomic. 2008 Nov;7(6):454-9. doi: 10.1093/bfgp/eln046. Epub 2008 Nov 21.
TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.
定向诱导基因组局部突变（TILLING）是一种反向遗传学策略，用于识别化学诱变群体中特定感兴趣基因的突变。TILLING于2000年首次被描述用于拟南芥中的突变检测，如今已应用于多种植物，包括大豆、水稻、大麦和玉米，以及动物模型系统，包括拟南芥、果蝇、秀丽隐杆线虫、大鼠、青鳉和斑马鱼，还用于发现人类自然发生的多态性。本综述总结了目前应用于斑马鱼的TILLING方法、斑马鱼群体中正在进行的TILLING项目和资源，以及斑马鱼TILLING的未来发展。
