利用定向诱导基因组局部突变技术在斑马鱼中进行反向遗传学研究。
Reverse genetics in zebrafish by TILLING.
作者信息
Moens Cecilia B, Donn Thomas M, Wolf-Saxon Emma R, Ma Taylur P
机构信息
HHMI and Division of Basic Science, Fred Hutchinson Cancer Research Center, B2-152, 1100 Fairview Ave. N., Seattle, WA 98109, USA.
出版信息
Brief Funct Genomic Proteomic. 2008 Nov;7(6):454-9. doi: 10.1093/bfgp/eln046. Epub 2008 Nov 21.
Abstract
TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.
摘要
定向诱导基因组局部突变（TILLING）是一种反向遗传学策略，用于识别化学诱变群体中特定感兴趣基因的突变。TILLING于2000年首次被描述用于拟南芥中的突变检测，如今已应用于多种植物，包括大豆、水稻、大麦和玉米，以及动物模型系统，包括拟南芥、果蝇、秀丽隐杆线虫、大鼠、青鳉和斑马鱼，还用于发现人类自然发生的多态性。本综述总结了目前应用于斑马鱼的TILLING方法、斑马鱼群体中正在进行的TILLING项目和资源，以及斑马鱼TILLING的未来发展。
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本文引用的文献
1
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Nucleic Acids Res. 2008 Jul;36(12):4047-56. doi: 10.1093/nar/gkn341. Epub 2008 Jun 3.
2
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases.利用工程化锌指核酸酶在斑马鱼中进行靶向基因失活。
Nat Biotechnol. 2008 Jun;26(6):695-701. doi: 10.1038/nbt1398. Epub 2008 May 25.
3
Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases.利用设计的锌指核酸酶在斑马鱼中进行可遗传的靶向基因破坏。
Nat Biotechnol. 2008 Jun;26(6):702-8. doi: 10.1038/nbt1409. Epub 2008 May 25.
4
TILLMore, a resource for the discovery of chemically induced mutants in barley.TILLMore，一个用于发现大麦化学诱导突变体的资源。
Plant Biotechnol J. 2008 Jun;6(5):477-85. doi: 10.1111/j.1467-7652.2008.00341.x. Epub 2008 Apr 15.
5
TILLING to detect induced mutations in soybean.利用定向诱导基因组局部突变技术检测大豆中的诱导突变
BMC Plant Biol. 2008 Jan 24;8:9. doi: 10.1186/1471-2229-8-9.
6
Genome-wide in situ exon capture for selective resequencing.用于选择性重测序的全基因组原位外显子捕获
Nat Genet. 2007 Dec;39(12):1522-7. doi: 10.1038/ng.2007.42. Epub 2007 Nov 4.
7
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Neuron. 2007 Aug 2;55(3):407-15. doi: 10.1016/j.neuron.2007.06.037.
8
Efficient genome-wide mutagenesis of zebrafish genes by retroviral insertions.通过逆转录病毒插入实现斑马鱼基因的高效全基因组诱变。
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9
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BMC Plant Biol. 2007 Apr 11;7:19. doi: 10.1186/1471-2229-7-19.
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Cell. 2007 Apr 6;129(1):69-82. doi: 10.1016/j.cell.2007.03.026.
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