Luci Carmelo, Reynders Ana, Ivanov Ivaylo I, Cognet Celine, Chiche Laurent, Chasson Lionel, Hardwigsen Jean, Anguiano Esperanza, Banchereau Jacques, Chaussabel Damien, Dalod Marc, Littman Dan R, Vivier Eric, Tomasello Elena
Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Institut National de la Santé et de la Recherche Médicale, U631, 13288 Marseille, France.
Nat Immunol. 2009 Jan;10(1):75-82. doi: 10.1038/ni.1681. Epub 2008 Nov 23.
NKp46+CD3- natural killer lymphocytes isolated from blood, lymphoid organs, lung, liver and uterus can produce granule-dependent cytotoxicity and interferon-gamma. Here we identify in dermis, gut lamina propria and cryptopatches distinct populations of NKp46+CD3- cells with a diminished capacity to degranulate and produce interferon-gamma. In the gut, expression of the transcription factor RORgammat, which is involved in the development of lymphoid tissue-inducer cells, defined a previously unknown subset of NKp46+CD3- lymphocytes. Unlike RORgammat- lamina propria and dermis natural killer cells, gut RORgammat+NKp46+ cells produced interleukin 22. Our data show that lymphoid tissue-inducer cells and natural killer cells shared unanticipated similarities and emphasize the heterogeneity of NKp46+CD3- cells in innate immunity, lymphoid organization and local tissue repair.
从血液、淋巴器官、肺、肝脏和子宫中分离出的NKp46⁺CD3⁻自然杀伤淋巴细胞可产生颗粒依赖性细胞毒性和干扰素-γ。在这里，我们在真皮、肠道固有层和隐窝斑块中鉴定出不同群体的NKp46⁺CD3⁻细胞，其脱颗粒和产生干扰素-γ的能力减弱。在肠道中，参与淋巴组织诱导细胞发育的转录因子RORγt的表达定义了一个以前未知的NKp46⁺CD3⁻淋巴细胞亚群。与RORγt⁻固有层和真皮自然杀伤细胞不同，肠道RORγt⁺NKp46⁺细胞产生白细胞介素22。我们的数据表明，淋巴组织诱导细胞和自然杀伤细胞具有意想不到的相似性，并强调了NKp46⁺CD3⁻细胞在固有免疫、淋巴组织形成和局部组织修复中的异质性。
