Kalimo K O, Ziola B R, Viljanen M K, Granfors K, Toivanen P
J Immunol Methods. 1977;14(2):183-95. doi: 10.1016/0022-1759(77)90008-4.
A solid-phase radioimmunoassay for detection of herpes simplex virus-specific IgG and IgM antibodies in human serum specimens is presented. Virus antigen is adsorbed on polystyrene balls and antibodies which attach to the antigen are detected by 125I-labeled antihuman-gamma or antihuman-mu immunoglobulins. A total of 76 specimens have been tested. The appearance of virus-specific IgG and IgM antibodies in primary herpetic infections was readily demonstrated. When serum samples from patients with past exposure to herpes simplex virus were tested, endpoint titers of virus-specific IgG antibodies were found to be 8 to 2048 times higher than titers determined by a complement fixation test. Apparent cross reactivity with varicella-zoster virus was observed in the present radioimmunoassay.
本文介绍了一种用于检测人血清标本中单纯疱疹病毒特异性IgG和IgM抗体的固相放射免疫测定法。病毒抗原吸附在聚苯乙烯球上，通过125I标记的抗人γ或抗人μ免疫球蛋白检测与抗原结合的抗体。共检测了76份标本。原发性疱疹感染中病毒特异性IgG和IgM抗体的出现很容易得到证实。当检测既往接触过单纯疱疹病毒患者的血清样本时，发现病毒特异性IgG抗体的终点滴度比补体结合试验测定的滴度高8至2048倍。在目前的放射免疫测定中观察到与水痘-带状疱疹病毒明显的交叉反应。
