Siefferman C M, Regelmann W E, Gray B H
Department of Microbiology, University of Minnesota, Minneapolis 55455.
Infect Immun. 1991 Jun;59(6):2152-7. doi: 10.1128/iai.59.6.2152-2157.1991.
The susceptibility of paired mucoid and nonmucoid variants of Pseudomonas aeruginosa isolated from 13 patients with cystic fibrosis (CF) to killing by a 55,000-Da bactericidal protein (BP55) from human polymorphonuclear leukocytes was studied. Mucoid and nonmucoid variants were equally sensitive to killing by BP55 at both pH 5.6 and pH 7.2. Eleven of the isolates were resistant to the bactericidal activity of 10% normal human serum but were as sensitive as the serum-sensitive isolates to BP55. Similarly, the 15 isolates with lipopolysaccharides (LPS) containing O-polysaccharide side chains (smooth LPS) were as sensitive to BP55 as those isolates with rough LPS.P. aeruginosa isolates from patients in poor clinical condition were more likely to have LPS of the smooth type and to be resistant to killing by 10% human serum than the isolates from patients in good clinical condition. We have concluded that the susceptibility of the P. aeruginosa isolates from patients with CF to killing by BP55 does not correlate with mucoid or nonmucoid variations, with the presence or absence of smooth LPS, or with the sensitivity or resistance to killing by normal human serum.
对从13例囊性纤维化（CF）患者中分离出的铜绿假单胞菌的成对黏液型和非黏液型变体，进行了其被人多形核白细胞的一种55,000道尔顿杀菌蛋白（BP55）杀伤的敏感性研究。黏液型和非黏液型变体在pH 5.6和pH 7.2时对BP55杀伤的敏感性相同。11株分离株对10%正常人血清的杀菌活性耐药，但对BP55的敏感性与血清敏感的分离株相同。同样，15株含有O-多糖侧链脂多糖（LPS）（光滑型LPS）的分离株对BP55的敏感性与粗糙型LPS的分离株相同。临床状况较差患者的铜绿假单胞菌分离株比临床状况良好患者的分离株更有可能具有光滑型LPS且对10%人血清杀伤耐药。我们得出结论，CF患者的铜绿假单胞菌分离株对BP55杀伤的敏感性与黏液型或非黏液型变异、光滑型LPS的有无或对正常人血清杀伤的敏感或耐药无关。
