Kita E, Sawaki M, Oku D, Hamuro A, Mikasa K, Konishi M, Emoto M, Takeuchi S, Narita N, Kashiba S
Department of Bacteriology, Nara Medical University, Japan.
J Antimicrob Chemother. 1991 Mar;27(3):273-84. doi: 10.1093/jac/27.3.273.
The effects of erythromycin stearate over a concentration range of 0.1-10 mg/l on production of elastase, protease and leucocidin by clinical isolates of Pseudomonas aeruginosa were investigated. Growth of P. aeruginosa N42 in broth was not affected significantly during 24 h culture with erythromycin (0.1-10 mg/l), although extracellular protein contents were reduced by erythromycin at concentrations of 0.1-1.0 mg/l. Production of elastase and protease by strain N42 was significantly suppressed by erythromycin with a maximum inhibition at 0.5 mg/l, but the complete inhibition of enzyme production was not achieved. In contrast, leucocidin production by strain N42 was completely impaired by erythromycin at concentrations of 0.1-5.0 mg/l. Although the leucotoxic activity, as determined by vital staining, was not detected, the leucocidin fraction prepared from the autolysate of strain N42 cultured with 10 mg/l of erythromycin induced morphological changes in human leucocytes, resulting in release of elastase. Erythromycin exerted similar effects on other clinical isolates of P. aeruginosa. These findings indicate that erythromycin might have a role in P. aeruginosa infection, although it has no direct antibacterial activity.
研究了硬脂酸红霉素在0.1 - 10 mg/l浓度范围内对铜绿假单胞菌临床分离株产生弹性蛋白酶、蛋白酶和杀白细胞素的影响。在含有红霉素（0.1 - 10 mg/l）的肉汤中培养24小时期间，铜绿假单胞菌N42的生长未受到显著影响，尽管在0.1 - 1.0 mg/l浓度的红霉素作用下细胞外蛋白质含量有所降低。N42菌株产生弹性蛋白酶和蛋白酶的能力被红霉素显著抑制，在0.5 mg/l时抑制作用最大，但未实现对酶产生的完全抑制。相比之下，在0.1 - 5.0 mg/l浓度的红霉素作用下，N42菌株产生杀白细胞素的能力完全受损。尽管通过活体染色未检测到杀白细胞毒性活性，但从用10 mg/l红霉素培养的N42菌株自溶产物中制备的杀白细胞素组分可诱导人白细胞形态变化，导致弹性蛋白酶释放。红霉素对其他铜绿假单胞菌临床分离株也有类似作用。这些发现表明，红霉素可能在铜绿假单胞菌感染中起作用，尽管它没有直接的抗菌活性。
