Scharmann W
J Gen Microbiol. 1976 Apr;93(2):283-91. doi: 10.1099/00221287-93-2-283.
A toxic substance, which destroyed leucocytes from man but was inactive against erythrocytes, was demonstrated in cultures of four out of 110 strains of Pseudomonas aeruginosa tested. The toxin, designated 'leucocidin', was cell-bound as a precursor toxin, exhibiting little or no toxicity. It was converted into toxin with maximum activity by various proteases including an endogenous elastase. The production of leucocidin was directly proportional to the number of bacteria and was not influenced by variations in media, iron concentration, pH or temperature. The best method for large-scale production of leucocidin was autolysis of washed bacteria.
在检测的110株铜绿假单胞菌中，有4株的培养物中发现了一种有毒物质，该物质能破坏人类白细胞，但对红细胞无活性。这种毒素被命名为“白细胞毒素”，它以前体毒素的形式与细胞结合，几乎没有或没有毒性。它被包括内源性弹性蛋白酶在内的各种蛋白酶转化为具有最大活性的毒素。白细胞毒素的产生与细菌数量成正比，不受培养基、铁浓度、pH值或温度变化的影响。大规模生产白细胞毒素的最佳方法是洗涤后的细菌自溶。
