Lemassu A, Lanéelle M A, Daffé M
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):171-5. doi: 10.1016/0378-1097(91)90153-2.
Nuclear magnetic resonance spectroscopy, fast-atom bombardment mass spectrometry as well as various chemical degradations and chromatographic techniques were used to re-examine the structure of a highly immunoreactive glycolipid previously described in Mycobacterium tuberculosis (strain Canetti) as a 2,3-diacyl trehalose 2'-sulfate (labelled SL-IV). Ion exchange chromatography allowed the recognition of a neutral and an acidic glycolipid, indistinguishable on conventional silica gel. The neutral glycolipid was shown to be serologically identical to SL-IV and its structure was established as 2,3-diacyl trehalose. It corresponded to the non-chemically defined highly observed immunoreactive lipid previously recognized by others in M. tuberculosis (H37Rv).
利用核磁共振光谱法、快原子轰击质谱法以及各种化学降解和色谱技术，对一种高度免疫反应性糖脂的结构进行了重新研究。该糖脂先前在结核分枝杆菌（卡内蒂菌株）中被描述为2,3 - 二酰基海藻糖2'-硫酸盐（标记为SL-IV）。离子交换色谱法能够识别出一种中性糖脂和一种酸性糖脂，它们在传统硅胶上无法区分。结果表明，该中性糖脂在血清学上与SL-IV相同，其结构确定为2,3 - 二酰基海藻糖。它与先前其他人在结核分枝杆菌（H37Rv）中识别出的未化学定义的高度免疫反应性脂质相对应。
