结核分枝杆菌中一种高度保守的免疫反应性脂质的证据。

Evidence for a highly conserved, immunoreactive lipid in Mycobacterium tuberculosis.

作者信息

Makonkawkeyoon S, Makonkawkeyoon L, Songsiri S, Dettrairat S

机构信息

Department of Clinical Immunology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand.

出版信息

Am Rev Respir Dis. 1989 Mar;139(3):774-8. doi: 10.1164/ajrccm/139.3.774.

DOI:10.1164/ajrccm/139.3.774

PMID:2466430

Abstract

A survey of the fractionated lipids of Mycobacterium tuberculosis H37Rv was conducted using rabbit antiserum raised to homologous and heterologous mycobacteria. One minor, highly apolar lipid was resolved by Florisil column chromatography, which reacted preferentially to anti-M. tuberculosis H37Rv rabbit antibodies. Other chromatographic properties, i.e., thin-layer chromatographic mobility and staining properties, suggested an analog of the phenolic glycolipid of Mycobacterium leprae. Preliminary results in the application of the glycolipid to tuberculous populations in northeast Thailand suggest a usefulness in screening for tuberculosis.

摘要

利用针对同源和异源分枝杆菌产生的兔抗血清，对结核分枝杆菌H37Rv的分级脂质进行了一项调查。一种少量的、高度非极性脂质通过弗罗里硅土柱色谱法分离出来，它优先与抗结核分枝杆菌H37Rv兔抗体发生反应。其他色谱特性，即薄层色谱迁移率和染色特性，表明它是麻风分枝杆菌酚糖脂的类似物。将该糖脂应用于泰国东北部结核人群的初步结果表明，它在结核病筛查中具有实用性。