Plotkowski M C, Chevillard M, Pierrot D, Altemayer D, Zahm J M, Colliot G, Puchelle E
Institut National de la Santé et de la Recherche Médicale U.314, Hôpital Maison Blanche, Reims, France.
J Clin Invest. 1991 Jun;87(6):2018-28. doi: 10.1172/JCI115231.
Human nasal polyps in outgrowth culture were used to study the Pseudomonas aeruginosa adhesion to respiratory cells. By scanning electron microscopy, P. aeruginosa were seen associated with ciliated cells, but by transmission electron microscopy, bacteria were never seen at the interciliary spaces or attached along cilia, but were identified trapped at the extremities of cilia, usually as bacterial aggregates. A fibronectin-containing fibrillar material was seen associated with aggregated bacteria. By time-lapse video microscopy, bacteria were seen to aggregate in the culture medium following their addition to the culture wells. Progressively, these aggregates were trapped by cilia or attached to migrating cells of a lower cell layer that protruded beneath the upper layer cells, at the outgrowth periphery. P. aeruginosa adhesion to these lower cell layer migrating cells was significantly higher than to ciliated or nonciliated cells of the upper cell layer. Migrating cells were intensely labeled by the complexes Con A and arachis hypogea agglutinin (PNA)-FITC, in contrast to the other cells. The percentage of PNA-labeled cells with attached bacteria was significantly higher than that without bacteria. These results suggest that changes of cell surface glycoconjugates related with cell migration may favor P. aeruginosa adhesion to respiratory cells.
利用体外培养的人鼻息肉研究铜绿假单胞菌对呼吸道细胞的黏附作用。通过扫描电子显微镜观察，可见铜绿假单胞菌与纤毛细胞相关联，但通过透射电子显微镜观察，未在纤毛间隙或沿纤毛附着处发现细菌，而是发现细菌被困在纤毛末端，通常呈细菌聚集体形式。可见一种含纤连蛋白的纤维状物质与聚集的细菌相关联。通过延时视频显微镜观察，发现细菌加入培养孔后在培养基中聚集。逐渐地，这些聚集体被纤毛捕获或附着于下层细胞的迁移细胞上，这些迁移细胞在上层细胞下方的生长边缘处突出。铜绿假单胞菌对这些下层细胞迁移细胞的黏附显著高于对上层细胞的纤毛细胞或非纤毛细胞的黏附。与其他细胞相比，迁移细胞被伴刀豆球蛋白A和花生凝集素（PNA）-异硫氰酸荧光素复合物强烈标记。黏附有细菌的PNA标记细胞的百分比显著高于未黏附细菌的细胞。这些结果表明，与细胞迁移相关的细胞表面糖缀合物变化可能有利于铜绿假单胞菌对呼吸道细胞的黏附。
