Pedersen Mikael E, Vestergaard Henrik T, Hansen Suzanne L, Bah Sekou, Diallo Drissa, Jäger Anna K
Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, 2 Universitetsparken, 2100 Copenhagen O, Denmark.
J Ethnopharmacol. 2009 Jan 30;121(3):472-5. doi: 10.1016/j.jep.2008.11.006. Epub 2008 Nov 14.
Several medicinal plants are used in Mali to treat epilepsy and convulsions. So far, no studies have investigated the pharmacological effect of these plants.
The aim of this study was to investigate the in vitro and in vivo antiepileptic potential of the ethanolic extracts of 11 Malian medicinal plants.
The extracts were screened for antiepileptic properties in the mouse cortical wedge preparation and in the [3H]-flumazenil binding assay. Two of the plant extracts were investigated for anticonvulsive properties in the pentylenetetrazol (PTZ) kindling model in mice. Possible side effects on motor impairment were evaluated using the rota-rod test.
Extracts of 10 of the 11 medicinal plants showed affinity to the benzodiazepine binding site on the GABAA receptor. Seven of the 11 extracts inhibited the spontaneous discharges (SEDs) in the mouse cortical wedge preparation, with the extracts of Flueggea virosa and Psorospermum senegalense being the most potent. However, when tested for in vivo anticonvulsive properties these two extracts failed to show any effect on PTZ-induced seizures in mice.
The pharmacological screening of the ethanolic extracts of 11 Malian medicinal plants in vitro lead to the identification of several extracts with potential anticonvulsant properties.
马里有几种药用植物用于治疗癫痫和惊厥。到目前为止，尚无研究调查这些植物的药理作用。
本研究旨在调查11种马里药用植物乙醇提取物的体外和体内抗癫痫潜力。
在小鼠皮质楔形标本和[3H] -氟马西尼结合试验中筛选提取物的抗癫痫特性。在小鼠戊四氮（PTZ）点燃模型中研究了两种植物提取物的抗惊厥特性。使用转棒试验评估对运动障碍的可能副作用。
11种药用植物中的10种提取物显示出对GABAA受体上苯二氮卓结合位点的亲和力。11种提取物中的7种在小鼠皮质楔形标本中抑制了自发放电（SEDs），其中余甘子和塞内加尔假虎刺的提取物效力最强。然而，在测试体内抗惊厥特性时，这两种提取物对小鼠PTZ诱导的癫痫发作未显示任何作用。
对11种马里药用植物乙醇提取物的药理筛选在体外鉴定出了几种具有潜在抗惊厥特性的提取物。
