Center for Environmental Nuclear Research, Directorate of Research, SRM University, Kattankulathur, Chennai, Tamil Nadu 603203, India.
BMC Complement Altern Med. 2014 Feb 22;14:70. doi: 10.1186/1472-6882-14-70.
Gymnema sylvestre is a highly valued ethno pharmacologically important medicinal plant used currently in many poly-herbal formulations due to its potential antidiabetic activity and other health benefits. The present study was carried out to analyze the anti-stress, anti-allergic, and antiulcer activity of the bioactive compounds present in Gymnema sylvestre leaves.
The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. The antioxidant activities were investigated using DPPH radical scavenging method. The characterization of the extract was carried out using standard compound by High Performance Thin Layer Chromatography (HPTLC) and phytochemical analysis in terms of total phenol, total flavonoids, reducing power and antioxidant potentials, etc. The in vivo studies on albino mice proved the purified fraction has anti-stress/anti-allergic activity against milk induced leucocytosis/eosinophilia and able to inhibit the aspirin induced gastric ulcers.
The quantitative estimation for aqueous extract exhibited total antioxidant (9.13 ± 0.04 μg/g), flavonoids (125.62 ± 26.84 μg/g), tannin (111.53 ± 15.13 μg/g), total phenol content (285.23 ± 1.11 μg/g) and free radical scavenging (52.14 ± 0.32%). Further the aqueous extract was consecutively purified by TLC and silica column chromatography. The purified fractions were characterized by HPTLC and GC-MS and the component was identified as gymnemic acid. The potency of the antimicrobial activity of the extract was studied with bacteria. Pharmacological experiments clearly demonstrated that the extracts of all plants given orally showed significant gastric protection against the asprin-induced gastric ulcer model in mice. Furthermore, healing effects were also confirmed through histopathological examination.
The aqueous extracts of the leaves of Gymnema sylvestre possess anti ulcerogenic, Anti allergic, Anti stress, properties that may be due to cytoprotective mechanism. These results support the ethno medical uses of the plant in the treatment of gastric ulcer.
武靴藤是一种具有高度价值的药用植物，在许多草药配方中被广泛使用，因为它具有潜在的抗糖尿病活性和其他健康益处。本研究旨在分析武靴藤叶中生物活性化合物的抗应激、抗过敏和抗溃疡活性。
对来自水提物的生物活性化合物进行初步的植物化学筛选，结果表明存在生物碱、三萜、类黄酮、甾体和皂苷。使用 DPPH 自由基清除法研究抗氧化活性。使用高效薄层色谱法（HPTLC）和总酚、总黄酮、还原能力和抗氧化潜力等植物化学分析对提取物进行标准化合物的表征。在白化小鼠中的体内研究证明，纯化部分具有抗应激/抗过敏活性，可对抗牛奶诱导的白细胞增多/嗜酸性粒细胞增多，并能抑制阿司匹林诱导的胃溃疡。
水提物的定量估计显示总抗氧化剂（9.13±0.04μg/g）、类黄酮（125.62±26.84μg/g）、单宁（111.53±15.13μg/g）、总酚含量（285.23±1.11μg/g）和自由基清除（52.14±0.32%）。进一步通过 TLC 和硅胶柱色谱法连续纯化水提取物。通过 HPTLC 和 GC-MS 对纯化部分进行了表征，成分被鉴定为武靴酸。研究了提取物对细菌的抗菌活性。药理实验清楚地表明，所有植物的提取物口服给药对小鼠阿司匹林诱导的胃溃疡模型具有显著的胃保护作用。此外，通过组织病理学检查也证实了愈合效果。
武靴藤叶的水提物具有抗溃疡、抗过敏、抗应激作用，这可能是由于细胞保护机制。这些结果支持该植物在治疗胃溃疡方面的民间医学用途。
