Aabenhus Rune, Stenram Unne, Andersen Leif Percival, Permin Henrik, Ljungh Asa
Department of Clinical Microbiology, National University Hospital (Rigshospitalet), Copenhagen, Denmark.
World J Gastroenterol. 2008 Dec 7;14(45):6954-9. doi: 10.3748/wjg.14.6954.
To infect mice with atypical Campylobacter concisus (C. concisus) for the first time.
Three separate experiments were conducted in order to screen the ability of five clinical C. concisus isolates of intestinal origin and the ATCC 33237 type strain of oral origin to colonize and produce infection in immunocompetent BALB/cA mice. The majority of the BALB/cA mice were treated with cyclophosphamide prior to C. concisus inoculation to suppress immune functions. Inoculation of C. concisus was performed by the gastric route.
C. concisus was isolated from the liver, ileum and jejunum of cyclophosphamide-treated mice in the first experiment. No C. concisus strains were isolated in the two subsequent experiments. Mice infected with C. concisus showed a significant loss of body weight from day two through to day five of infection but this decreased at the end of the first week. Histopathological examination did not consistently find signs of inflammation in the gut, but occasionally microabscesses were found in the liver of infected animals.
Transient colonization with C. concisus was observed in mice with loss of body weight. Future studies should concentrate on the first few days after inoculation and in other strains of mice.
首次用非典型简明弯曲杆菌（C. concisus）感染小鼠。
进行了三项独立实验，以筛选五株肠道来源的临床简明弯曲杆菌分离株和一株口腔来源的ATCC 33237标准菌株在免疫功能正常的BALB/cA小鼠中定殖并产生感染的能力。大多数BALB/cA小鼠在接种简明弯曲杆菌之前用环磷酰胺处理以抑制免疫功能。通过胃途径接种简明弯曲杆菌。
在第一个实验中，从环磷酰胺处理的小鼠的肝脏、回肠和空肠中分离出简明弯曲杆菌。在随后的两个实验中未分离到简明弯曲杆菌菌株。感染简明弯曲杆菌的小鼠在感染的第2天至第5天体重显著减轻，但在第一周结束时体重减轻情况有所缓解。组织病理学检查并未始终发现肠道有炎症迹象，但在感染动物的肝脏中偶尔发现微脓肿。
在体重减轻的小鼠中观察到简明弯曲杆菌的短暂定殖。未来的研究应集中在接种后的头几天以及其他品系的小鼠身上。
