Kumar Rajendran Senthil, Arunachalam Sankaralingam
School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Eur J Med Chem. 2009 May;44(5):1878-83. doi: 10.1016/j.ejmech.2008.11.001. Epub 2008 Nov 12.
Some water-soluble polymer-copper(II) complexes, [Cu(phen)(l-phe)(BPEI)]ClO(4).4H(2)O (phen=1,10-phenanthroline, l-phe=l-phenylalanine, and BPEI=branched polyethyleneimine), with various amounts of copper(II) chelates in the polymer chain, were prepared by ligand substitution method in water-ethanol medium and characterized by infra-red, UV-visible, EPR spectral and elemental analysis methods. Binding of these complexes with calf thymus DNA (CT DNA) has been investigated by absorption spectroscopy, emission spectroscopy and gel electrophoresis techniques. The experimental results indicate that the amount of copper(II) chelate content in the polymer backbone have marked effect on the binding affinity to CT DNA. Interactions like electrostatic interaction, van der Waals interaction, hydrogen bonding and/or partial intercalation binding modes exist in this system. A sample of polymer-copper(II) complex was tested for its antibacterial and antifungal activity against certain human pathogenic organisms and it was found to have good antibacterial and antifungal activities.
通过配体取代法在水 - 乙醇介质中制备了一些水溶性聚合物 - 铜（II）配合物，即[Cu(phen)(l - phe)(BPEI)]ClO₄·4H₂O（phen = 1,10 - 菲咯啉，l - phe = L - 苯丙氨酸，BPEI = 支化聚乙烯亚胺），其聚合物链中含有不同量的铜（II）螯合物，并通过红外、紫外 - 可见、电子顺磁共振光谱和元素分析方法对其进行了表征。利用吸收光谱、发射光谱和凝胶电泳技术研究了这些配合物与小牛胸腺DNA（CT DNA）的结合情况。实验结果表明，聚合物主链中铜（II）螯合物的含量对其与CT DNA的结合亲和力有显著影响。该体系中存在静电相互作用、范德华相互作用、氢键和/或部分嵌入结合模式等相互作用。对一种聚合物 - 铜（II）配合物样品针对某些人类致病生物体的抗菌和抗真菌活性进行了测试，发现其具有良好的抗菌和抗真菌活性。
