Zeevi Ben Yosef Dafna, Ben-Dov Eitan, Kushmaro Ariel
Department of Biotechnology Engineering and National Institute for Biotechnology, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2704-9. doi: 10.1099/ijs.0.65462-0.
A bacterial strain, designated RS.Sph.026(T), was isolated from mucus of the coral Fungia granulosa collected from the northern Red Sea (Gulf of Eilat, Israel). The bacterium was found to be Gram-negative, non-motile, halotolerant and heterotrophic. Comparative 16S rRNA gene sequence analyses showed that strain RS.Sph.026(T) belonged to the order Rhizobiales, with the highest levels of 16S RNA gene sequence similarity with Rhodobium orientis (92 %). Strain RS.Sph.026(T) grew optimally at a salinity of 3-4 %, pH 7.5-8 and 25-30 degrees C. The major cellular fatty acids were cis-7-octadecenoic acid (C(18 : 1)omega7small es, Cyrillic; 57.2 %) and C(19 : 0) cyclo omega8small es, Cyrillic (15.5 %). The DNA G+C content of strain RS.Sph.026(T) was 67.1 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain RS.Sph.026(T) represents a novel genus and species in the order Rhizobiales, for which the name Amorphus coralli gen. nov., sp. nov. is proposed. The type strain is RS.Sph.026(T) (=LMG 24307(T)=DSM 19760(T)).
从红海北部（以色列埃拉特湾）采集的颗粒状角孔珊瑚黏液中分离出一株细菌，命名为RS.Sph.026(T)。该细菌为革兰氏阴性、无运动性、耐盐且异养型。16S rRNA基因序列比较分析表明，RS.Sph.026(T)菌株属于根瘤菌目，与东方红球菌的16S rRNA基因序列相似度最高（92%）。RS.Sph.026(T)菌株在盐度3 - 4%、pH值7.5 - 8以及25 - 30℃条件下生长最佳。主要细胞脂肪酸为顺式-7-十八碳烯酸（C(18 : 1)ω7，西里尔文；57.2%）和C(19 : 0)环ω8，西里尔文（15.5%）。RS.Sph.026(T)菌株的DNA G + C含量为67.1 mol%。基于其表型特征和系统发育独特性，RS.Sph.026(T)菌株代表根瘤菌目中一个新的属和种，为此提出新属名Amorphus coralli gen. nov.，新种名Amorphus coralli sp. nov.。模式菌株为RS.Sph.026(T)（=LMG 24307(T)=DSM 19760(T)）。
