Jiang Cheng-Ying, Liu Ying, Liu Yan-Yang, You Xiao-Yan, Guo Xu, Liu Shuang-Jiang
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2898-903. doi: 10.1099/ijs.0.2008/000562-0.
A novel mesophilic and acidophilic Gram-positive bacterium, designated strain TC-34(T), was isolated from solfataric soil. Strain TC-34(T) grew aerobically at 17-40 degrees C and pH 2.0-6.0, and optimally at 28 degrees C and pH 3.0. Analysis based on 16S rRNA gene sequences showed that strain TC-34(T) was phylogenetically related to members of the genus Alicyclobacillus, with the highest similarity (94.8 %) to Alicyclobacillus pomorum. Strain TC-34(T) showed a range of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including growth temperature, assimilation of carbon sources and production of acids from a range of compounds. Strain TC-34(T) was able to oxidize ferrous iron and its growth was correlated with the oxidation of Fe(2+) in culture medium. omega-Alicyclic fatty acids were not detected. On the basis of these results, it was concluded that strain TC-34(T) represents a novel species of the genus Alicyclobacillus, for which the name Alicyclobacillus ferrooxydans (type strain TC-34(T)=JCM 15090(T)=CGMCC 1.6357(T)) is proposed.
从硫磺土壤中分离出一株新型嗜温嗜酸革兰氏阳性菌，命名为TC-34(T)菌株。TC-34(T)菌株在17-40℃和pH 2.0-6.0条件下好氧生长，最适生长温度为28℃，最适pH为3.0。基于16S rRNA基因序列的分析表明，TC-34(T)菌株在系统发育上与 Alicyclobacillus 属的成员相关，与 Alicyclobacillus pomorum 的相似性最高（94.8%）。TC-34(T)菌株表现出一系列表型特征，使其与已确认的 Alicyclobacillus 菌种区分开来，包括生长温度、碳源同化以及从一系列化合物中产酸。TC-34(T)菌株能够氧化亚铁离子，其生长与培养基中Fe(2+)的氧化相关。未检测到ω-脂环族脂肪酸。基于这些结果，得出结论：TC-34(T)菌株代表 Alicyclobacillus 属的一个新物种，为此提出名为 Alicyclobacillus ferrooxydans（模式菌株TC-34(T)=JCM 15090(T)=CGMCC 1.6357(T)）。
