Wang Hong, Lehtomäki Annimari, Tolvanen Katariina, Puhakka Jaakko, Rintala Jukka
Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland.
Bioresour Technol. 2009 Apr;100(7):2311-5. doi: 10.1016/j.biortech.2008.10.040. Epub 2008 Dec 4.
The bacterial communities in three continuously stirred tank reactors co-digesting cow manure with grass silage, oat straw, and sugar beet tops, respectively, were investigated by 16S rRNA gene-based fingerprints and clone libraries. The analyses revealed both clearly distinct and similar phylotypes in the bacterial communities between the reactors. The major groups represented in the three reactors were Clostridia, unclassified Bacteria, and Bacteroidetes. Phylotypes affiliated with Bacilli or Deltaproteobacteria were unique to the sugar beet and straw reactor, respectively. Unclassified Bacteria dominated in sugar beet reactor while in the straw and grass reactor Clostridia was the dominant group. An increase in organic loading rate from 2 to 3kg volatile solids m(-3) d(-1) resulted in larger changes in the bacterial community in the straw compared to grass reactor. The study shed more light on the evolution of bacterial community during anaerobic co-digestion of different crops and manure to methane.
分别用三个连续搅拌槽式反应器对牛粪与青贮草料、燕麦秸秆和甜菜叶进行共消化处理，通过基于16S rRNA基因的指纹图谱和克隆文库对其中的细菌群落进行了研究。分析结果显示，各反应器细菌群落中的系统发育型既有明显差异，也有相似之处。三个反应器中的主要菌群为梭菌纲、未分类细菌和拟杆菌门。与芽孢杆菌或δ-变形菌相关的系统发育型分别在甜菜和秸秆反应器中独有。未分类细菌在甜菜反应器中占主导地位，而在秸秆和草料反应器中梭菌纲是优势菌群。有机负荷率从2千克挥发性固体/立方米·天增加到3千克挥发性固体/立方米·天，相比草料反应器，秸秆反应器中的细菌群落变化更大。该研究进一步揭示了不同作物与粪便厌氧共消化产生甲烷过程中细菌群落的演变情况。
