Patrick-Iwuanyanwu K C, Wegwu M O, Ayalogu E O
Department of Biochemistry, Toxicological Unit, University of Port Harcourt, P.M.B. 5323, Choba, Port Harcourt, Rivers State, Nigeria.
Pak J Biol Sci. 2007 Feb 15;10(4):617-21. doi: 10.3923/pjbs.2007.617.621.
The hepatoprotective effects of garlic (Allium sativum), ginger (Zingiber officinale) and vitamin E pre-treatment against carbon tetrachloride (CCl4)-induced liver damage in male wistar albino rats were investigated. Carbon tetrachloride (0.5 mL kg(-1) body weight) was administered after 28 days of feeding animals with diets containing ginger, garlic, vitamin E and various mixtures of ginger and garlic. Serum alanine amino transferase, aspartate amino transferase and alkaline phosphatase levels, 24 h after CCl4 administration, decreased significantly (p < or = 0.05) in rats pre-treated with garlic, ginger, vitamin E and various mixtures of garlic and ginger than in CCl4-treated rats only. Lipid peroxidation expressed by serum malondialdehyde (MDA) concentration was assayed to assess the extent of liver damage by CCl4; including the extent of hepatoprotection by garlic, ginger and vitamin E. MDA concentration was significantly decreased (p < or = 0.05) in rats pretreated with garlic, ginger, vitamin E and various mixtures of garlic and ginger than in rats administered CCl4-alone. Histological examination of the liver revealed severe infiltration of inflammatory cells in rats treated with CCl4 alone. However, the observed alteration in the normal architecture of the hepatic cells decreased remarkably in pre-treated rats.
研究了大蒜（蒜）、生姜（姜）和维生素E预处理对雄性Wistar白化大鼠四氯化碳（CCl4）诱导的肝损伤的保肝作用。在用含生姜、大蒜、维生素E以及生姜和大蒜各种混合物的饲料喂养动物28天后，给予四氯化碳（0.5 mL kg(-1)体重）。在给予CCl4 24小时后，与仅用CCl4处理的大鼠相比，用大蒜、生姜、维生素E以及大蒜和生姜各种混合物预处理的大鼠血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶水平显著降低（p≤0.05）。通过检测血清丙二醛（MDA）浓度来评估脂质过氧化，以评估CCl4对肝脏的损伤程度；包括大蒜、生姜和维生素E的保肝程度。与仅给予CCl4的大鼠相比，用大蒜、生姜、维生素E以及大蒜和生姜各种混合物预处理的大鼠MDA浓度显著降低（p≤0.05）。肝脏组织学检查显示，仅用CCl4处理的大鼠有严重的炎性细胞浸润。然而，在预处理的大鼠中，肝细胞正常结构的观察到的改变明显减少。
