Rosa C A R, Keller K M, Keller L A M, González Pereyra M L, Pereyra C M, Dalcero A M, Cavaglieri L R, Lopes C W G
Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Instituto de Veterinária, Rodovia BR 465Km 7, Seropédica 23890-000, Rio de Janeiro, Brazil.
Toxicon. 2009 Feb;53(2):283-8. doi: 10.1016/j.toxicon.2008.11.015. Epub 2008 Dec 6.
Mycotoxin contamination of animal feeds represents a hazard to human and animal health due to potential transmission to meat and milk. Barley by-products are alternative feeding supplies for animal production. The aims of this assay were to study the mycobiota of feedstuffs and finished swine feed, to determine the ability of Aspergillus and Penicillium isolates to produce ochratoxin A (OTA) and to evaluate OTA occurrence in these substrates. Corn, brewers' grains and finished swine feed samples were collected from different factories. Fungal counts were higher than 2.8x10(4)CFU g(-1). Fusarium, Aspergillus and Penicillium genera were isolated at high levels. A 23.7% of the isolates produced 9-116 microg kg(-1) of OTA in vitro. Corn samples (44%) were contaminated with 42-224 microg kg(-1) of OTA. Finished feed (31%) and brewers' grains samples (13%) were contaminated with 36-120 microg kg(-1) and 28-139 microg kg(-1) of OTA, respectively. This is the first scientific report on contamination by OTA-producer molds and OTA in swine feedstuffs from Brazil. The presence of OTA in raw materials and finished feed requires periodic monitoring to prevent mycotoxicoses in animal production, reduce economic losses and minimize hazards to human health.
动物饲料中的霉菌毒素污染会对人类和动物健康构成危害，因为其有可能传播到肉类和奶类中。大麦副产品是动物生产的替代饲料来源。本试验的目的是研究饲料原料和成品猪饲料中的真菌菌群，确定曲霉属和青霉属分离株产生赭曲霉毒素A（OTA）的能力，并评估这些底物中OTA的存在情况。从不同工厂采集了玉米、啤酒糟和成品猪饲料样本。真菌计数高于2.8×10⁴CFU g⁻¹。镰刀菌属、曲霉属和青霉属的分离率很高。23.7%的分离株在体外产生了9 - 116μg kg⁻¹的OTA。玉米样本（44%）被42 - 224μg kg⁻¹的OTA污染。成品饲料（31%）和啤酒糟样本（13%）分别被36 - 120μg kg⁻¹和28 - 139μg kg⁻¹的OTA污染。这是关于巴西猪饲料中OTA产生菌霉菌和OTA污染的第一份科学报告。原料和成品饲料中OTA的存在需要定期监测，以预防动物生产中的霉菌毒素中毒，减少经济损失，并将对人类健康的危害降至最低。
Zotero 插件