在俄罗斯远东地区分离和鉴定汉坦病毒及该地区肾综合征出血热的病因。
Isolation and characterization of hantaviruses in Far East Russia and etiology of hemorrhagic fever with renal syndrome in the region.
机构信息
Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
出版信息
Am J Trop Med Hyg. 2012 Mar;86(3):545-53. doi: 10.4269/ajtmh.2012.11-0297.
Hemorrhagic fever with renal syndrome (HFRS) is a serious public health issue in Far East Russia. Two different hantaviruses were isolated from rodents captured in the Khabarovsk region: Amur virus (AMRV; Khekhtsir/AP209/2005 strain from Apodemus peninsulae) and Hantaan virus (HTNV; Galkino/AA57/2002 strain from A. agrarius). Genetic analysis of the new isolates revealed that the M and L segments were apparently different between AMRV and HTNV, but S segments of the two viruses were closer. The antigenicities of AMRV, HTNV, and Seoul virus (SEOV) were differentiated by cross-neutralization. Serological differential diagnoses of 67 HFRS patients in the Prymorsky and Khabarovsk regions of Far East Russia were conducted using a neutralization test. The results revealed that the major cause of HFRS varied with location in Far East Russia: SEOV for Vladivostok city in the Prymorsky region, AMRV in rural areas of the Primorsky region, and probably HTNV for the Khabarovsk region.
肾综合征出血热（HFRS）是俄罗斯远东地区的一个严重公共卫生问题。从哈巴罗夫斯克地区捕获的啮齿动物中分离到两种不同的汉坦病毒：阿穆尔病毒（AMRV；来自半岛田鼠的 Khekhtsir/AP209/2005 株）和汉坦病毒（HTNV；来自黑线姬鼠的 Galkino/AA57/2002 株）。对新分离株的遗传分析表明，AMRV 和 HTNV 之间的 M 和 L 片段明显不同，但两种病毒的 S 片段更接近。通过交叉中和试验区分了 AMRV、HTNV 和首尔病毒（SEOV）的抗原性。使用中和试验对俄罗斯远东地区普里莫尔斯基和哈巴罗夫斯克地区的 67 例 HFRS 患者进行了血清学鉴别诊断。结果表明，HFRS 的主要病因因地理位置而异：普里莫尔斯基地区的符拉迪沃斯托克市为 SEOV，普里莫尔斯基地区农村地区为 AMRV，哈巴罗夫斯克地区可能为 HTNV。
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