Beck Relja, Beck Ana, Kusak Josip, Mihaljević Zeljko, Lucinger Snjezana, Zivicnjak Tatjana, Huber Duro, Gudan Andrea, Marinculić Albert
Faculty of Veterinary Medicine, Department for Parasitology and Parasitic Diseases, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
Vet Parasitol. 2009 Feb 23;159(3-4):308-11. doi: 10.1016/j.vetpar.2008.10.068. Epub 2008 Nov 1.
The aim of the present study was to investigate the prevalence of Trichinella infection in wolves (Canis lupus) in a 17,468 km(2) area in Croatia. Muscle samples were collected from 67 wolves between 1996 and 2007 and analyzed by artificial digestion. Muscle larvae were detected in 21 wolves (31%) and genotyped by multiplex PCR. Trichinella britovi was the predominant species confirmed in 90% (19 wolves) while Trichinella spiralis was detected in 9% (2 wolves). The presence of the so called "domestic"Trichinella species was a surprise since, to date, only T. britovi had been reported in wild animals in this region. The larval burdens in infected animals ranged from 0.3 to 45.9 larvae per gram. The prevalence of infected animals varied by geographic region; infected animals were found in the region of Gorski Kotar (20%) which has very similar environment to the region of Lika, where almost all wolves were found infected. Interestingly, this is the first report of infected wolves in Dalmatia.
本研究的目的是调查克罗地亚一个面积为17468平方公里区域内狼（犬属狼种）旋毛虫感染的流行情况。在1996年至2007年间从67只狼身上采集肌肉样本，并通过人工消化法进行分析。在21只狼（31%）中检测到肌肉幼虫，并通过多重聚合酶链反应进行基因分型。布氏旋毛虫是主要的确诊物种，占90%（19只狼），而旋毛形线虫占9%（2只狼）。所谓“家养”旋毛虫物种的存在令人惊讶，因为迄今为止，该地区野生动物中仅报告过布氏旋毛虫。受感染动物体内的幼虫负荷为每克0.3至45.9条幼虫。受感染动物的流行率因地理区域而异；在戈尔什基科塔尔地区发现了受感染动物（20%），该地区的环境与利卡地区非常相似，而利卡地区几乎所有狼都被发现受到感染。有趣的是，这是达尔马提亚地区狼感染的首次报告。
