改良BD GeneOhm耐甲氧西林金黄色葡萄球菌检测方法用于从合并的鼻腔、皮肤和咽喉样本中检测耐甲氧西林金黄色葡萄球菌的一年经验。
One-year experience with modified BD GeneOhm MRSA assay for detection of methicillin-resistant Staphylococcus aureus from pooled nasal, skin, and throat samples.
作者信息
Svent-Kucina Natasa, Pirs Mateja, Mueller-Premru Manica, Cvitkovic-Spik Vesna, Kofol Romina, Seme Katja
机构信息
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
出版信息
Diagn Microbiol Infect Dis. 2009 Feb;63(2):132-9. doi: 10.1016/j.diagmicrobio.2008.10.005. Epub 2008 Dec 10.
We report our 1-year experience with modified GeneOhm MRSA assay (formerly IDI-MRSA) for pooled surveillance specimens in low methicillin-resistant Staphylococcus aureus (MRSA) prevalence clinical setting. We have successfully modified the GeneOhm MRSA assay protocol during the specimen preparation step by adding an extra washing step followed by pooling of up to 3 samples per patient (nose, skin, with or without throat) at the lysis step. The sensitivity of the modified assay compared with conventional cultivation was 94.3%, specificity 99.2%, negative predictive value 99.2%, and positive predictive value 94.3%. The modified test is reliable and performed well compared with conventional culture methods in our clinical setting with low-level prevalence of MRSA colonization. Our findings support the use of pooling of the patients samples as a cost-effective way of screening for MRSA colonization.
我们报告了在耐甲氧西林金黄色葡萄球菌（MRSA）低流行率临床环境中，使用改良的GeneOhm MRSA检测法（原IDI-MRSA）对混合监测标本进行检测的1年经验。我们在标本制备步骤中成功改良了GeneOhm MRSA检测方案，增加了一个额外的洗涤步骤，然后在裂解步骤将每位患者的多达3份样本（鼻子、皮肤，有或无咽喉样本）进行混合。与传统培养法相比，改良检测法的灵敏度为94.3%，特异性为99.2%，阴性预测值为99.2%，阳性预测值为94.3%。在我们这个MRSA定植率较低的临床环境中，与传统培养方法相比，改良检测法可靠且表现良好。我们的研究结果支持将患者样本混合作为一种经济有效的MRSA定植筛查方法。
Zotero 插件