Li Hong, Chen Vicky, Chen Yanwen, Baumgartner J Craig, Machida Curtis A
Department of Endodontology, Oregon Health & Science University, School of Dentistry, Portland, Oregon 97239, USA.
J Endod. 2009 Jan;35(1):23-9. doi: 10.1016/j.joen.2008.09.017. Epub 2008 Oct 31.
Irreversible pulpitis and apical periodontitis are inflammatory diseases caused by opportunistic bacteria with possible co-infection with latent herpesviruses. The objectives of this study are to identify herpesviruses, including human cytomegalovirus (HCMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV-1), and Varicella zoster virus (VZV) in patients with irreversible pulpitis (n = 29) or apical periodontitis, either primary (n = 30) or previously treated (n = 23). Using primary and nested polymerase chain reaction (PCR) and reverse transcription-PCR, EBV DNA and RNA were present in endodontic pathoses in significantly higher percentages (43.9% and 25.6%, respectively) compared with healthy pulp controls (0% and 0%, respectively). HCMV DNA and RNA were found in measurable numbers in both endodontic patients (15.9% and 29.3%, respectively) and in healthy pulp controls (42.1% and 10.5%, respectively). HSV-1 DNA was found in low percentages in endodontic patients (13.4%), and only one patient showed the presence of VZV. In conclusion, EBV may be associated with irreversible pulpitis and apical periodontitis.
不可逆性牙髓炎和根尖周炎是由机会性细菌引起的炎症性疾病，可能伴有潜伏性疱疹病毒的合并感染。本研究的目的是在不可逆性牙髓炎患者（n = 29）或根尖周炎患者（原发性，n = 30；或既往已治疗，n = 23）中鉴定疱疹病毒，包括人巨细胞病毒（HCMV）、爱泼斯坦-巴尔病毒（EBV）、单纯疱疹病毒1型（HSV-1）和水痘-带状疱疹病毒（VZV）。使用巢式聚合酶链反应（PCR）和逆转录PCR，与健康牙髓对照（分别为0%和0%）相比，EBV DNA和RNA在牙髓病损中的存在比例显著更高（分别为43.9%和25.6%）。在牙髓病患者（分别为15.9%和29.3%）和健康牙髓对照（分别为42.1%和10.5%）中均检测到可测量数量的HCMV DNA和RNA。HSV-1 DNA在牙髓病患者中的发现比例较低（13.4%），仅1例患者显示存在VZV。总之，EBV可能与不可逆性牙髓炎和根尖周炎有关。
