Institute of Microbiology Prof Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
J Endod. 2011 Jan;37(1):10-6. doi: 10.1016/j.joen.2010.09.009. Epub 2010 Nov 13.
It has been suggested that viruses, especially herpesviruses, can play a role in the pathogenesis of marginal and apical periodontitis. This study aimed to detect herpesviruses types 1 to 8, namely herpes simplex virus (HSV-1/2), varicella zoster virus (VZV), Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus-6 (HHV-6), human herpesvirus-7 (HHV-7), and human herpesvirus-8 (HHV-8) as well as human papillomavirus (HPV) in acute apical abscesses.
Twenty-four samples were taken by aspiration of the purulent exudate from acute apical abscesses. DNA extracted from clinical samples served as a template in single or nested polymerase chain reaction (PCR) assays for the detection of the target viruses.
Control PCR reactions with β-globin gene primers revealed that all samples but one had detectable human DNA. Of the 23 abscess samples positive for the β-globin gene, 14 (61%) were positive for at least one of the target human viruses. Thirteen (56.5%) cases had herpesvirus: HHV-8 occurred in 11 (48%), VZV and HHV-6B in two (9%), and HHV-7 and HSV-1/2 in one (4%). EBV and HCMV were not present in any of the examined samples. HPV was detected in three (13%) abscess samples. Viral coinfection was found in five cases, with one case harboring three of the targeted viruses.
A large number of abscess samples were positive for at least one target virus. Unexpectedly, HHV-8 was for the first time detected and in a high prevalence. Papillomavirus and other herpesviruses were also found for the first time in endodontic abscesses. Although these findings suggest an association, the specific role of viruses in the pathogenesis of acute apical abscesses awaits further clarification.
有人提出，病毒，尤其是疱疹病毒，可能在边缘性和根尖周炎的发病机制中起作用。本研究旨在检测 1 型至 8 型疱疹病毒，即单纯疱疹病毒（HSV-1/2）、水痘带状疱疹病毒（VZV）、EB 病毒（EBV）、人巨细胞病毒（HCMV）、人疱疹病毒-6（HHV-6）、人疱疹病毒-7（HHV-7）和人疱疹病毒-8（HHV-8）以及人乳头瘤病毒（HPV）在急性根尖脓肿中的存在情况。
通过抽吸急性根尖脓肿的脓性渗出物采集 24 个样本。从临床样本中提取的 DNA 作为模板，用于单重或嵌套聚合酶链反应（PCR）检测以检测目标病毒。
用β-珠蛋白基因引物进行对照 PCR 反应，结果显示所有样本均有可检测的人 DNA。在 23 个β-珠蛋白基因阳性的脓肿样本中，有 14 个（61%）至少有一种目标人类病毒呈阳性。13 例（56.5%）有疱疹病毒：HHV-8 发生在 11 例（48%）中，VZV 和 HHV-6B 发生在 2 例（9%）中，HHV-7 和 HSV-1/2 各发生在 1 例（4%）中。在检测的样本中均未发现 EBV 和 HCMV。在 3 个（13%）脓肿样本中检测到 HPV。5 例存在病毒合并感染，1 例存在 3 种目标病毒。
大量脓肿样本至少对一种目标病毒呈阳性。出乎意料的是，HHV-8 首次被检测到且阳性率较高。乳头瘤病毒和其他疱疹病毒也首次在牙髓脓肿中被发现。尽管这些发现表明存在关联，但病毒在急性根尖脓肿发病机制中的具体作用仍有待进一步阐明。
