Rana S V, Mehta S
Department of Gastroenterology, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
Indian J Exp Biol. 1991 Mar;29(3):259-62.
Twelve young rhesus monkeys weighing 1.5-2.0 kg were acclimatised for a month in the animal house of the Institute. After acclimatization 6 monkeys were placed on a protein calorie deficient diet and remaining 6 on normal diet. The deficiency state was indicated by reduction in body weight, significant decrease in serum albumin, lack of physical activity and shedding of fur. At this stage, animals of both groups were sacrificed and brains were removed. Cerebrum was separated, blotted on filter paper and weighed. In vitro incorporation of (U-C14)-glucose was done for 2 hr in approximately 300 mg cerebrum. Incorporation of this precursor was determined in cerebrum, lipids, its fractions and proteins in both groups. There was increased incorporation of (U-C14)-glucose into tissue, gangliosides, free fatty acids and esterified cholesterol but no significant change in myelin marker lipids and proteins in PCM group was noticed as compared to age matched controls.
12只体重为1.5 - 2.0千克的幼年恒河猴在该研究所的动物房适应环境一个月。适应环境后，6只猴子被置于蛋白质热量缺乏的饮食中，其余6只给予正常饮食。体重减轻、血清白蛋白显著降低、缺乏体力活动和掉毛表明出现了缺乏状态。在此阶段，两组动物均被处死并取出大脑。分离出大脑，用滤纸吸干并称重。在约300毫克大脑中进行了2小时的（U - C14）-葡萄糖体外掺入实验。测定了两组大脑、脂质、其组分和蛋白质中这种前体的掺入情况。与年龄匹配的对照组相比，PCM组中（U - C14）-葡萄糖在组织、神经节苷脂、游离脂肪酸和酯化胆固醇中的掺入增加，但髓磷脂标记脂质和蛋白质未发现显著变化。
