Basu Samar, Helmersson Johanna, Jarosinska Dorota, Sällsten Gerd, Mazzolai Barbarra, Barregård Lars
Oxidative Stress and Inflammation, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala Science Park, and Center of Excellence-Inflammation, Uppsala University Hospital, Uppsala, Sweden.
Free Radic Res. 2009 Jan;43(1):85-91. doi: 10.1080/10715760802610851.
Oxidative stress is assumed to be the key underlying factor in the pathogenesis of many common diseases. This study describes the basal levels of 8-iso-PGF(2alpha ), a major F(2)-isoprostane and an in vivo oxidative stress biomarker in healthy subjects from three countries, namely Italy, Poland and Sweden, in relation to their smoking habits, age and gender. It studied urinary 8-iso-PGF(2alpha ) in 588 subjects from Sweden (n=220), Italy (n=203) and Poland (n=165). Polish subjects had the highest levels of F(2)-isoprostanes followed by the Swedish and Italians when adjusted for smoking, age, sex and creatinine and the inter-country differences were statistically significant. Smokers had significantly higher levels of 8-iso-PGF(2alpha ) compared to non-smokers in all countries and there was a moderate decrease with age. Women had only slightly lower 8-iso-PGF(2alpha ) than men. There is a difference in F(2)-isoprostane levels in vivo between countries. Smoking, age and gender affect isoprostane formation and should be taken into consideration in clinical studies of oxidative stress.
氧化应激被认为是许多常见疾病发病机制中的关键潜在因素。本研究描述了来自意大利、波兰和瑞典这三个国家的健康受试者体内主要的F(2)-异前列腺素8-异前列腺素F2α（8-iso-PGF(2α)）的基础水平，它是一种体内氧化应激生物标志物，并探讨了其与吸烟习惯、年龄和性别的关系。该研究对来自瑞典（n=220）、意大利（n=203）和波兰（n=165）的588名受试者的尿液8-异前列腺素F2α进行了检测。在对吸烟、年龄、性别和肌酐进行校正后，波兰受试者的F(2)-异前列腺素水平最高，其次是瑞典人和意大利人，国家间差异具有统计学意义。在所有国家，吸烟者的8-异前列腺素F2α水平均显著高于非吸烟者，且随着年龄增长有适度下降。女性的8-异前列腺素F2α水平仅略低于男性。不同国家间体内F(2)-异前列腺素水平存在差异。吸烟、年龄和性别会影响异前列腺素的形成，在氧化应激的临床研究中应予以考虑。
