葡萄糖和转化生长因子β2调节培养的人视网膜周细胞的活力及其血管内皮生长因子的释放。

Glucose and TGFbeta2 modulate the viability of cultured human retinal pericytes and their VEGF release.

作者信息

Vidro Eileen K, Gee Stephen, Unda Richard, Ma Jian-xing, Tsin Andrew

机构信息

Department of Biology, University of Texas at San Antonio, San Antonio, Texas 78249, USA.

出版信息

Curr Eye Res. 2008 Nov;33(11):984-93. doi: 10.1080/02713680802450976.

PURPOSE

Determine the effects of glucose and exogenous TGFbeta2 on viability and VEGF release by human retinal pericytes (HRP).

METHODS

Human retinal pericytes (HRP) were cultured in 5 mM (physiologic) or high (18 mM) glucose with or without added TGFbeta2. Viable cells were counted; TGFbeta2 and VEGF in the conditioned media (CM) were measured by ELISA.

RESULTS

High glucose significantly reduced viable cell number and increased the levels of TGFbeta2 and VEGF. TGFbeta2 caused a significant dose-dependent effect on viable cell number and on the level of VEGF secreted into the CM by HRP in physiologic glucose, decreasing viable cell number, and increasing VEGF release per 1000 cells at a low concentration (0.1 ng/ml) and increasing viable cell number and decreasing VEGF release per 1000 cells at higher concentrations (1.0 and 10 ng/ml). TGFbeta2 affected neither parameter in high glucose.

CONCLUSIONS

Elevated glucose decreased HRP viability and modulated changes in TGFbeta2 and VEGF release. This suggests a novel mechanism for HRP dropout in diabetic retinopathy.

目的

确定葡萄糖和外源性转化生长因子β2（TGFβ2）对人视网膜周细胞（HRP）活力及血管内皮生长因子（VEGF）释放的影响。

方法

将人视网膜周细胞（HRP）培养于5 mM（生理浓度）或高浓度（18 mM）葡萄糖中，添加或不添加TGFβ2。对活细胞进行计数；通过酶联免疫吸附测定法（ELISA）检测条件培养基（CM）中的TGFβ2和VEGF。

结果

高糖显著减少活细胞数量，并增加TGFβ2和VEGF水平。在生理浓度葡萄糖条件下，TGFβ2对HRP的活细胞数量以及分泌到CM中的VEGF水平产生显著的剂量依赖性影响，低浓度（0.1 ng/ml）时降低活细胞数量并增加每1000个细胞的VEGF释放量，高浓度（1.0和10 ng/ml）时增加活细胞数量并减少每1000个细胞的VEGF释放量。在高糖环境中，TGFβ2对这两个参数均无影响。