Lange W R, Ball J C, Adler W H, Brown E, Pyle R, Hoffman W, Dax E M
Public Health Service, National Institute on Drug Abuse's Addiction Research Center, Baltimore, MD 21224.
Public Health Rep. 1991 Jul-Aug;106(4):451-5.
Serum specimens obtained from a nationwide sample of parenteral drug abusers (PDAs) during the period 1971-72 had previously been screened for human immunodeficiency virus (HIV) antibodies. Some specimens were considered to be positive to both ELISA and Western blot (WB) analysis. These findings have been a topic of controversy, since HIV was not thought to have penetrated at-risk populations at such an early date. This study was a followup of those PDAs with apparent seropositivity to WB analysis. Of 10 persons followed, only one death (in 1985) was documented, and postmortem findings were inconsistent with HIV infection. Eight of the remaining PDAs were traced and found to be alive and well in 1989. Fresh specimens were obtained from the two persons with the strongest 1971-72 WB staining and were found to be both ELISA and WB negative on retesting. Their T-cell parameters were within normal limits. We concluded that the earlier WB results were most likely false positives and that definitive evidence of HIV infection in the U.S. addict population as early as 1971-72 is still lacking.
1971年至1972年期间，从全国范围内的非肠道药物滥用者（PDA）样本中采集的血清标本，此前已进行过人类免疫缺陷病毒（HIV）抗体筛查。一些标本被认为ELISA和蛋白质印迹法（WB）分析均呈阳性。这些发现一直存在争议，因为人们认为HIV在如此早的时期尚未侵入高危人群。本研究是对那些WB分析显示明显血清阳性的PDA进行的随访。在随访的10人中，仅记录到1例死亡（1985年），尸检结果与HIV感染不符。其余8名PDA在1989年被追踪到，发现他们还活着且健康状况良好。从1971年至1972年WB染色最强的两人身上获取了新鲜标本，重新检测发现ELISA和WB均为阴性。他们的T细胞参数在正常范围内。我们得出结论，早期的WB结果很可能是假阳性，并且仍缺乏1971年至1972年美国吸毒人群中HIV感染的确切证据。
