Matsushita Kenshi, Uchiyama Jumpei, Kato Shin-ichiro, Ujihara Takako, Hoshiba Hiroshi, Sugihara Shigeyoshi, Muraoka Asako, Wakiguchi Hiroshi, Matsuzaki Shigenobu
Department of Pediatrics, Kochi Medical School, Nankoku, Kochi, Japan.
FEMS Microbiol Lett. 2009 Feb;291(2):201-8. doi: 10.1111/j.1574-6968.2008.01455.x. Epub 2008 Dec 11.
Increases in multidrug-resistant strains of Serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. In the search for bacteriophages to control infectious diseases caused by multidrug-resistant S. marcescens, three phages (KSP20, KSP90, and KSP100) were isolated from environmental water and were characterized morphologically and genetically. KSP20 and KSP90 belonged to morphotype A1 of the family Myoviridae, and KSP100 belonged to morphotype C3 of the family Podoviridae. Analysis of the DNA region coding virion proteins, together with their morphological features, indicated that KSP20, KSP90, and KSP100 were related to the P2-like phage (temperate), T4-type phage (virulent), and phiEco32 phage (virulent), respectively. Based on amino acid sequences of the major capsid protein, KSP90 formed a new branch with a Stenotrophomonas maltophilia phage, Smp14, in the T4-type phage phylogeny. Both Smp14 and phiEco32 have been reported as potential therapeutic phages. These results suggest that KSP90 and KSP100 may be candidate therapeutic phages to control S. marcescens infection.
粘质沙雷氏菌多重耐药菌株的增加在儿科领域引起了极大关注，尤其是在新生儿重症监护病房。在寻找用于控制多重耐药粘质沙雷氏菌引起的传染病的噬菌体时，从环境水中分离出三种噬菌体（KSP20、KSP90和KSP100），并对其进行了形态学和遗传学特征分析。KSP20和KSP90属于肌尾噬菌体科的A1形态型，KSP100属于短尾噬菌体科的C3形态型。对编码病毒粒子蛋白的DNA区域的分析及其形态特征表明，KSP20、KSP90和KSP100分别与P2样噬菌体（温和型）、T4型噬菌体（烈性）和phiEco32噬菌体（烈性）相关。基于主要衣壳蛋白的氨基酸序列，KSP90在T4型噬菌体系统发育中与嗜麦芽窄食单胞菌噬菌体Smp14形成了一个新分支。Smp14和phiEco32都已被报道为潜在的治疗性噬菌体。这些结果表明，KSP90和KSP100可能是控制粘质沙雷氏菌感染的候选治疗性噬菌体。
