Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences, Kathmandu, Nepal.
Department of Clinical Laboratory Services, Manmohan Memorial Medical College and Teaching Hospital, Kathmandu, Nepal.
Biomed Res Int. 2017;2017:3723254. doi: 10.1155/2017/3723254. Epub 2017 Nov 22.
Bacteriophages are being the subject of interest for alternative antimicrobial therapy for infectious diseases in recent years. Therapeutic effectiveness regarding phage therapy is a matter of concern since it is the most promising biological treatment of this era. Hence, the present study was aimed to isolate the potential bacteriophages present in river water samples and to analyze their host range among clinical strains of bacteria. Ten different locations of Kathmandu valley were selected for the collection of river water for the detection of probable phages. Bacteriophages were isolated from water samples using the double agar overlay method. Isolated phages were purified by diluting in the SM-buffer and filtering through 0.22 m filter. Purified lysate was further processed for analyzing its host range by using spot method. Their host range was characterized against 20 bacterial strains, including multidrug-resistant. Total 67 different phages were isolated against 8 different host organisms. Out of them, forty-seven phages were selected for analyzing its host range. Among them, phages (ΦSER) had the broad host range infecting 17 different bacterial strains including multidrug-resistant harboring ESBL and MBL genotypes. However, phages (ΦKP) had narrow host range in comparison to other phages. Isolated phages had the potential effect against clinical strains of bacteria along with their broader host spectrum. Most importantly, promising effect against MDR pathogens in this study has raised the probable chances of the utility of these phages for biological control of bacterial infection including MBL and ESBL strains.
近年来，噬菌体作为治疗传染病的替代抗菌疗法引起了人们的兴趣。噬菌体治疗的疗效是一个值得关注的问题，因为它是这个时代最有前途的生物治疗方法。因此，本研究旨在从河流水样中分离潜在的噬菌体，并分析它们在临床细菌菌株中的宿主范围。选择加德满都山谷的 10 个不同地点采集河水样本，以检测可能存在的噬菌体。使用双层琼脂覆盖法从水样中分离噬菌体。用 SM 缓冲液稀释分离的噬菌体，并用 0.22μm 过滤器过滤进行纯化。纯化的裂解物进一步通过点法分析其宿主范围。对其宿主范围进行了表征，共针对 20 种细菌菌株（包括多药耐药菌）。总共分离出 67 种不同的噬菌体针对 8 种不同的宿主生物。其中，选择了 47 种噬菌体来分析其宿主范围。其中，噬菌体（ΦSER）具有广泛的宿主范围，可感染包括携带 ESBL 和 MBL 基因型的 17 种不同细菌菌株。相比之下，噬菌体（ΦKP）的宿主范围比其他噬菌体窄。分离出的噬菌体对临床细菌菌株具有潜在的作用，同时具有更广泛的宿主谱。最重要的是，本研究中对 MDR 病原体的有希望的效果提高了这些噬菌体用于包括 MBL 和 ESBL 菌株在内的细菌感染的生物控制的可能性。
