Jardim Júnior Elerson Gaetti, Bosco Joseane Maria Dias, Lopes Angélica Marquezim, Landucci Luís Fernando, Jardim Ellen Cristina Gaetti, Carneiro Sílvia Rosana Soares
Department of Pathology, Propedeutic Clinics, Araçatuba Dental School, State University of São Paulo.
J Appl Oral Sci. 2006 Jun;14(3):153-6. doi: 10.1590/s1678-77572006000300001.
The aim of this study was to determine the frequency of isolation of Actinobacillus actinomycetemcomitans (Aa) in 100 patients with chronic periodontitis, 14 patients with aggressive periodontitis, 142 pre-school children with gingivitis and 134 periodontally healthy subjects. Samples of subgingival plaque were taken using sterilized paper points introduced into periodontal pockets or gingival crevice for 60 seconds and inoculated on TSBV agar, which was incubated under anaerobiosis at 37 degrees C, for 4 days. Microbial identification was performed through biochemical methods and morphocellular and morphocolonial analysis. Aa was detected in 40.3% of healthy subjects, 68% of patients with chronic periodontitis, 92.86% of patients with aggressive periodontitis and 40.14% of children with gingivitis. The rate of recovery of Aa in the tested human groups proved to be higher than previously reported and in agreement with participation of this facultative anaerobe as a member of native microbiota of the periodontium and its relation with aggressive and chronic periodontitis in Brazil.
本研究的目的是确定100例慢性牙周炎患者、14例侵袭性牙周炎患者、142例患牙龈炎的学龄前儿童以及134例牙周健康受试者中伴放线放线杆菌（Aa）的分离频率。使用无菌纸尖插入牙周袋或龈沟60秒采集龈下菌斑样本，并接种于TSBV琼脂上，于37℃厌氧条件下培养4天。通过生化方法以及形态细胞和形态菌落分析进行微生物鉴定。在40.3%的健康受试者、68%的慢性牙周炎患者、92.86%的侵袭性牙周炎患者以及40.14%的患牙龈炎儿童中检测到了Aa。在受试人群中Aa的检出率高于先前报道，这与该兼性厌氧菌作为牙周天然微生物群成员的参与情况及其与巴西侵袭性和慢性牙周炎的关系相符。
