Kamma J J, Diamanti-Kipioti A, Nakou M, Mitsis F J
Department of Periodontology, School of Dental Medicine, University of Athens, Greece.
J Periodontal Res. 2000 Feb;35(1):33-41. doi: 10.1034/j.1600-0765.2000.035001033.x.
Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. Subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). The samples were cultured for bacterial isolation anaerobically and in 10% CO2 plus air using selective and non-selective media. Forty-one different microbial species were isolated. Gemella morbillorum and Peptostreptococcus magnus were statistically significantly more frequently detected in incisors while P. micros, Streptococcus intermedius, Bacteroides forsythus, Fusobacterium nucleatum, Prevotella loeschei, P. melaninogenica and Selenomonas sputigena were more frequently detected in molars. The bacterial species S. constellatus, G. morbillorum and P. magnus were isolated in greater numbers in incisors and P. micros, S. intermedius, Campylobacter concisus, Bacteroides egertheii, B. forsythus, P. oralis and S. sputigena were isolated in greater numbers in molars, respectively. Cluster analysis revealed 4 clusters in which 6-7 bacterial species were elevated above mean levels. Cluster I was predominated by S. constellatus, S. mitis, S. sanguis, G. morbillorum, P. melaninogenica and P. oralis; cluster II was predominated by S. sanguis, Actinomyces naeslundii, Capnocytophaga gingivalis, C. ochracea and P. intermedia; cluster III was predominated by S. mitis, C. ochracea, F. nucleatum, P. loeschei, P. melaninogenica and P. oralis; and finally cluster IV was predominated by S. sanguis, C. gingivalis, Veillonella parvula, Campylobacter gracilis, F. nucleatum and P. intermedia. The bacterial species S. constellatus, P. micros, Pseudoramibacter alactolyticus, Eikenella corrodens and F. nucleatum were associated with non-bleeding sites while S. intermedius, C. concisus, P. intermedia and P. loescheii were found more frequently in bleeding sites.
乳牙萌出通过为细菌定植提供适宜的生态位，对口腔环境有很大影响。本研究旨在调查40名4至5岁全身健康儿童的乳切牙、乳尖牙和乳磨牙龈下微生物群的组成，这些儿童为随机选取。龈下菌斑样本取自乳切牙（61、81）、乳尖牙（53、73）和乳磨牙（64、84）的近中颊侧部位。样本在厌氧条件下以及在10%二氧化碳加空气环境中，使用选择性和非选择性培养基进行细菌分离培养。共分离出41种不同的微生物。在切牙中，麻疹孪生球菌和大消化链球菌的检出率在统计学上显著更高，而微小消化链球菌、中间链球菌、福赛斯坦纳菌、具核梭杆菌、洛氏普雷沃菌、产黑色素普雷沃菌和龈沟栖瘤胃球菌在磨牙中更常被检出。星座链球菌、麻疹孪生球菌和大消化链球菌在切牙中的分离数量更多，而微小消化链球菌、中间链球菌、简明弯曲菌、埃氏拟杆菌、福赛斯坦纳菌、口腔普雷沃菌和龈沟栖瘤胃球菌在磨牙中的分离数量分别更多。聚类分析揭示了4个聚类，其中6 - 7种细菌的水平高于平均水平。聚类I以星座链球菌、轻链球菌、血链球菌、麻疹孪生球菌、产黑色素普雷沃菌和口腔普雷沃菌为主；聚类II以血链球菌、内氏放线菌、牙龈二氧化碳嗜纤维菌、黄褐二氧化碳嗜纤维菌和中间普雷沃菌为主；聚类III以轻链球菌、黄褐二氧化碳嗜纤维菌、具核梭杆菌、洛氏普雷沃菌、产黑色素普雷沃菌和口腔普雷沃菌为主；最后聚类IV以血链球菌、牙龈二氧化碳嗜纤维菌、小韦荣球菌、纤细弯曲菌、具核梭杆菌和中间普雷沃菌为主。星座链球菌、微小消化链球菌、解乳糖假瘤胃杆菌、腐蚀埃肯菌和具核梭杆菌与无出血部位相关，而中间链球菌、简明弯曲菌、中间普雷沃菌和洛氏普雷沃菌在出血部位更常被发现。
