Joshi Vinayak Mahableshwar, Bhat Kishore Gajanan, Kugaji Manohar Suresh, Ingalgi Preeti Shivaji
Department of Molecular Biology and Immunology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.
J Indian Soc Periodontol. 2016 Mar-Apr;20(2):141-4. doi: 10.4103/0972-124X.175171.
Aggregatibacter actinomycetemcomitans (Aa), an important primary periodontal pathogen, is known for its strong virulence characteristics that cause periodontal disease. We investigated Aa occurrence in Indian individuals using culture and 16 s rDNA polymerase chain reaction (PCR).
A cross-sectional study with 100 participants each in the healthy and chronic periodontitis (CP) groups was conducted. The subgingival plaque was collected and immediately plated on selective media for Aa. The remaining plaque samples were used for DNA extraction. PCR was performed using specific primers for Aa.
The detection of bacteria and the clinical parameters between the groups were compared using the Mann-Whitney U-test. For assessing the agreement between the results of anaerobic culture and PCR, Kappa analyses were performed.
Aa levels using culture and PCR was 51% and 69% in the CP group and 12% and 30% in the healthy group, respectively. The two groups showed significant differences (P < 0.00001). The detection accuracy of culture and PCR was assessed, and the coefficient of accuracy (k) was highly significant in the healthy (0.3103; P < 0.0001) and CP groups (0.1536; P < 0.0497).
Aa was predominantly found in the CP group compared with the healthy group, which is consistent with previous findings. Our results showed that both techniques can be used for detecting Aa. An ideal technique for detecting subgingival microorganisms should be carefully selected depending on the scope of the intended future work.
伴放线聚集杆菌(Aa)是一种重要的原发性牙周病原体,以其导致牙周疾病的强毒力特征而闻名。我们使用培养和16 s rDNA聚合酶链反应(PCR)研究了印度个体中Aa的存在情况。
进行了一项横断面研究,健康组和慢性牙周炎(CP)组各有100名参与者。收集龈下菌斑并立即接种在用于Aa的选择性培养基上。其余菌斑样本用于DNA提取。使用针对Aa的特异性引物进行PCR。
使用曼-惠特尼U检验比较两组之间细菌的检测情况和临床参数。为评估厌氧培养和PCR结果之间的一致性,进行了kappa分析。
CP组中使用培养法和PCR法检测到的Aa水平分别为51%和69%,健康组分别为12%和30%。两组显示出显著差异(P < 0.00001)。评估了培养法和PCR法的检测准确性,健康组(0.3103;P < 0.0001)和CP组(0.1536;P < 0.0497)的准确性系数(k)非常显著。
与健康组相比,CP组中Aa的检出率更高,这与先前的研究结果一致。我们的结果表明,两种技术均可用于检测Aa。应根据未来预期工作的范围谨慎选择检测龈下微生物的理想技术。