涎腺多形性腺瘤中的腱生蛋白和纤连蛋白。

Tenascin and fibronectin in pleomorphic adenoma of the salivary gland.

作者信息

Bento Patrícia Meira, Freitas Roseana de Almeida, Pinto Leão Pereira, de Souza Lélia Batista

机构信息

Campina Grande Dental School, State University of Paraíba, Campina Grande, PB, Brazil.

出版信息

J Appl Oral Sci. 2006 Jun;14(3):198-202. doi: 10.1590/s1678-77572006000300010.

DOI:10.1590/s1678-77572006000300010

PMID:19089073

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4327197/

Abstract

OBJECTIVES

To analyze the expression and distribution pattern of extracellular matrix components in pleomorphic adenomas of the major and minor salivary glands and to compare the morphological findings of these tumors with the immunohistochemical expression, considering the different types of stroma predominating in each case.

METHODS AND RESULTS

The expression of tenascin (TN) and fibronectin (FN) was analyzed in 23 cases of pleomorphic adenomas, 11 major and 12 minor salivary gland tumors, by the streptavidin-biotin method using anti-tenascin and anti-fibronectin antibodies. In addition, the immunohistochemical results were correlated with the morphological findings of the lesions. All cases analyzed were immunoreactive for the antibodies used. Fibronectin showed strong labeling in fibrous and chondroid stroma, while labeling was weak in hyaline and myxoid stroma. Tenascin expression was more intense in fibrous and chondroid stroma and moderate in hyaline and myxoid stroma.

CONCLUSIONS

No difference in the expression of these proteins was observed between major and minor salivary gland tumors.

摘要

目的

分析大、小涎腺多形性腺瘤中细胞外基质成分的表达及分布模式，并结合各病例中占主导地位的不同类型基质，比较这些肿瘤的形态学表现与免疫组化表达情况。

方法与结果

采用抗腱生蛋白和抗纤连蛋白抗体，通过链霉亲和素-生物素法，分析了23例多形性腺瘤（11例大涎腺肿瘤和12例小涎腺肿瘤）中腱生蛋白（TN）和纤连蛋白（FN）的表达。此外，将免疫组化结果与病变的形态学表现相关联。所有分析病例对所用抗体均呈免疫反应性。纤连蛋白在纤维性和软骨样基质中显示强标记，而在透明样和黏液样基质中标记较弱。腱生蛋白在纤维性和软骨样基质中的表达更强，在透明样和黏液样基质中为中度表达。