新型隐球菌抗原成分的分离与纯化。
Isolation and purification of antigenic components of Cryptococcus.
作者信息
Wozniak Karen L, Levitz Stuart M
机构信息
Department of Medicine and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
出版信息
Methods Mol Biol. 2009;470:71-83. doi: 10.1007/978-1-59745-204-5_7.
Abstract
The encapsulated fungal pathogens Cryptococcus neoformans and Cryptococcus gattii are significant agents of life-threatening infections, particularly in persons with suppressed cell-mediated immunity. This chapter provides detailed methodology for the purification of two of the major antigen fractions of C. neoformans: glucuronoxylomannan (GXM) and mannoprotein (MP). GXM is the primary component of the polysaccharide capsule, which is the major cryptococcal virulence factor. In contrast, MPs have been identified as key antigens that stimulate T-cell responses. Purification of GXM and MP should assist investigators studying the antigenic, biochemical, and virulence properties of Cryptococcus species.
摘要
被膜包裹的真菌病原体新型隐球菌和格特隐球菌是危及生命感染的重要病原体，尤其是在细胞介导免疫受到抑制的人群中。本章提供了纯化新型隐球菌两种主要抗原成分的详细方法：葡糖醛酸木甘露聚糖（GXM）和甘露蛋白（MP）。GXM是多糖荚膜的主要成分，而多糖荚膜是隐球菌的主要毒力因子。相比之下，MP已被确定为刺激T细胞反应的关键抗原。GXM和MP的纯化应有助于研究人员研究隐球菌属的抗原性、生化特性和毒力特性。
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