Ribeiro D A, Matsumoto M A
Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.
J Oral Rehabil. 2008 Dec;35(12):925-33. doi: 10.1111/j.1365-2842.2008.01891.x.
Nowadays, selective cyclooxygenase-2 non-steroidal anti-inflammatory drugs have been largely used in surgical practice for reducing oedema and pain. However, the association between these drugs and laser therapy is not known up to now. Herein, the aim of this study was to evaluate the action of anti-COX-2 selective drug (celecoxib) on bone repair associated with laser therapy. A total of 64 rats underwent surgical bone defects in their tibias, being randomly distributed into four groups: Group 1) negative control; Group 2) animals treated with celecoxib; Group 3) animals treated with low-level power laser and Group 4) animals treated with celecoxib and low-level power laser. The animals were killed after 48 h, 7, 14 and 21 days. The tibias were removed for morphological, morphometric and immunohistochemistry analysis for COX-2. Statistical significant differences (P < 0.05) were observed in the quality of bone repair and quantity of formed bone between groups at 14 days after surgery for Groups 3 and 4. COX-2 immunoreactivity was more intense in bone cells for intermediate periods evaluated in the laser-exposed groups. Taken together, such results suggest that low-level laser therapy is able to improve bone repair in the tibia of rats as a result of an up-regulation for cyclooxygenase-2 expression in bone cells.
如今，选择性环氧化酶-2非甾体抗炎药已在外科手术中广泛用于减轻水肿和疼痛。然而，到目前为止，这些药物与激光治疗之间的关联尚不清楚。在此，本研究的目的是评估抗COX-2选择性药物（塞来昔布）对与激光治疗相关的骨修复的作用。总共64只大鼠在其胫骨上进行了手术性骨缺损，随机分为四组：第1组）阴性对照组；第2组）用塞来昔布治疗的动物；第3组）用低功率激光治疗的动物；第4组）用塞来昔布和低功率激光治疗的动物。在48小时、7天、14天和21天后处死动物。取出胫骨进行形态学、形态计量学和COX-2免疫组织化学分析。在术后14天，第3组和第4组之间在骨修复质量和形成骨的数量方面观察到统计学显著差异（P < 0.05）。在激光照射组评估的中间时期，骨细胞中的COX-2免疫反应性更强。综上所述，这些结果表明，低水平激光治疗能够通过上调骨细胞中环氧化酶-2的表达来改善大鼠胫骨的骨修复。
