College of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
J Biomed Mater Res A. 2009 Dec 15;91(4):953-63. doi: 10.1002/jbm.a.32240.
The synthesis and characterization of a thermosensitive chitosan/poly(vinyl alcohol) (PVA) hydrogel containing hydroxyapatite for protein delivery were first reported. Two synthetic processes were introduced, that is, in situ and ex situ routes. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and rheological analysis were employed to characterize the hydrogel. The gel formation decreased the crystallinity of the hydroxyapatite crystal. FTIR results showed that the hydroxyapatite crystals were partially substituted by carbonate. It was supposed that the structural features of hydroxyapatite were close to those of biological apatites. The thermosensitive property of the gels was evaluated by rheological analysis, which indicated that the strength of hydroxyapatite-chitosan/PVA composite gels was notably enhanced in comparison with that of pure chitosan/PVA gel, especially for hydroxyapatite-chitosan/PVA composite gels synthesized through the in situ process. The performances of composite gels containing different amounts of hydroxyapatite were further studied. For the hydroxyapatite-chitosan/PVA composite gels containing 0.1 mM hydroxyapatite synthesized through in situ process, the swelling degree was the lowest, and the speed of protein release was the slowest. Therefore, the composite gels are attractive for applications as protein delivery, artificial bones, and scaffolds for tissue engineering.
首次报道了一种含羟基磷灰石的温敏性壳聚糖/聚乙烯醇（PVA）水凝胶的合成与表征，用于蛋白质递送。介绍了两种合成方法，即原位法和异位法。采用傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和流变分析对水凝胶进行了表征。凝胶形成降低了羟基磷灰石晶体的结晶度。FTIR 结果表明，羟基磷灰石晶体部分被碳酸根取代。据推测，羟基磷灰石的结构特征与生物磷灰石相似。通过流变分析评估了凝胶的温敏性，结果表明，与纯壳聚糖/PVA 凝胶相比，羟基磷灰石-壳聚糖/PVA 复合凝胶的强度明显增强，尤其是通过原位法合成的羟基磷灰石-壳聚糖/PVA 复合凝胶。进一步研究了含有不同含量羟基磷灰石的复合凝胶的性能。对于通过原位法合成的含有 0.1mM 羟基磷灰石的羟基磷灰石-壳聚糖/PVA 复合凝胶，其溶胀度最低，蛋白质释放速度最慢。因此，这些复合凝胶作为蛋白质递送、人工骨和组织工程支架具有吸引力。
