Lambeth L S, Yu M, Anderson D E, Crameri G, Eaton B T, Wang L-F
CSIRO Livestock Industries, Australian Animal Health Laboratory, PO Bag 24, Geelong, VIC 3220, Australia.
Arch Virol. 2009;154(2):199-207. doi: 10.1007/s00705-008-0287-3. Epub 2008 Dec 23.
Nariva virus (NarPV) was isolated from forest rodents (Zygodontomys b. brevicauda) in eastern Trinidad in the early 1960s. Initial classification within the family Paramyxoviridae was based mainly on morphological observations including the structure of nucleocapsids and virion surface projections. Here, we report the characterization of the complete genome sequence of NarPV. The genome is 15,276 nucleotides in length, conforming to the rule-of-six, and has a genome organization typical of most members of the family, with six transcriptional units in the order 3'-N-P-M-F-H-L-5'. The gene junctions contain highly conserved gene start and stop signals and a tri-nucleotide intergenic sequence present in most members of the subfamily Paramyxovirinae. Sequence comparison studies indicate that NarPV is most closely related to Mossman virus, which was isolated from wild rats (Rattus leucopus) in Queensland, Australia, in 1970. This study confirmed the classification of NarPV as a member of the subfamily Paramyxovirinae and established the close genome organization and sequence relationship between the two rodent paramyxoviruses isolated almost a decade apart and from two locations separated by more than 15,000 km.
纳里瓦病毒（NarPV）于20世纪60年代初在特立尼达岛东部的森林啮齿动物（短尾稻鼠）中分离得到。最初在副粘病毒科内的分类主要基于形态学观察，包括核衣壳结构和病毒粒子表面突起。在此，我们报告纳里瓦病毒完整基因组序列的特征。该基因组长度为15276个核苷酸，符合六规则，具有该科大多数成员典型的基因组结构，有六个转录单元，顺序为3'-N-P-M-F-H-L-5'。基因连接处包含高度保守的基因起始和终止信号以及副粘病毒亚科大多数成员中存在的三核苷酸基因间序列。序列比较研究表明，纳里瓦病毒与1970年在澳大利亚昆士兰州从野生大鼠（白足鼠）中分离得到的莫斯曼病毒关系最为密切。这项研究证实了纳里瓦病毒作为副粘病毒亚科成员的分类，并确立了这两种相隔近十年、来自相距超过15000公里的两个地点的啮齿动物副粘病毒之间紧密的基因组结构和序列关系。
