Asgary Saeed, Kamrani Farshid Akbari
Iranian Center for Endodontic Research, Dental Research Center, Dental School, Shahid Beheshti University MC, Tehran, Iran.
J Oral Sci. 2008 Dec;50(4):469-74. doi: 10.2334/josnusd.50.469.
The aim of this in vitro study was to assess the antibacterial effects of gray and white mineral trioxide aggregate (GMTA and WMTA), calcium hydroxide (CH), Portland cement (PC) and a new endodontic cement (NEC) on various species of microorganisms, using agar diffusion test. A base layer of Petri plates was made using Muller-Hinton agar. Five cavities were made in agar and filled with fresh mixed materials after 24 h. Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and a mixture of these bacteria were seeded by pour plate. The plates were preincubated for 2 h at room temperature followed by incubation at 37 degrees C. The inhibition zone diameters were measured at 24, 48 and 72 h. The highest mean diameters of growth inhibition zones were observed around NEC and CH. According to one-way ANOVA, there was a significant difference among test groups (P < 0.001), while post-hoc test revealed no significant difference between the mean zone diameters of NEC and CH, and also between MTAs and PC. However, there was a significant difference between CH and NEC in comparison with MTAs and PC groups (P < 0.001). It appears that NEC may act as a potent antibacterial agent similar to CH.
本体外研究的目的是使用琼脂扩散试验评估灰色和白色三氧化矿物凝聚体（GMTA和WMTA）、氢氧化钙（CH）、波特兰水泥（PC）和一种新型牙髓水泥（NEC）对各种微生物的抗菌作用。用Muller-Hinton琼脂制作培养皿的底层。在琼脂中制作五个空洞，24小时后用新鲜混合材料填充。通过倾注平板接种铜绿假单胞菌、粪肠球菌、金黄色葡萄球菌、大肠杆菌以及这些细菌的混合物。平板在室温下预孵育2小时，然后在37℃下孵育。在24、48和72小时测量抑菌圈直径。在NEC和CH周围观察到生长抑制圈的最高平均直径。根据单因素方差分析，试验组之间存在显著差异（P < 0.001），而事后检验显示NEC和CH的平均抑菌圈直径之间以及MTA和PC之间没有显著差异。然而，与MTA和PC组相比，CH和NEC之间存在显著差异（P < 0.001）。似乎NEC可能作为一种与CH类似的强效抗菌剂发挥作用。
